Fazilah Ariffin, Azemi Mohamed N Mohd, Karim Alias A, Norakma Mohd N
School of Industrial Technology, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
J Agric Food Chem. 2009 Feb 25;57(4):1527-31. doi: 10.1021/jf8028013.
Hemicelluloses from oil palm frond (OPF) were extracted using 3 M potassium hydroxide (KOH) for 4 h at 40 degrees C with stirring at 400 rpm to obtain hemicelluloses A and B. The total yield of the hemicellulose isolated from OPF was 33% (dry weight). Both hemicelluloses A and B were then subjected to hydrothermal treatment at 121 degrees C and 1.03 x 10(5) Pa for 10, 30, and 50 min. Physicochemical characterizations of hydrothermally treated hemicelluloses, such as Klason lignin content and reducing sugar content, were performed to study the effect of autohydrolysis processing on OPF-derived hemicelluloses. It was shown that Klason lignin content in hemicellulose A was higher than that in hemicellulose B and decreased after hydrothermal treatment. Hydrothermal treatment enhanced the solubility of hemicelluloses, which reflects their higher reducing sugar content. Monosaccharide analysis using HPLC showed that xylose was the predominant monosaccharide for both hemicelluloses A and B.
采用3M氢氧化钾（KOH）在40℃下以400rpm搅拌4小时，从油棕叶（OPF）中提取半纤维素，以获得半纤维素A和B。从OPF中分离出的半纤维素的总产率为33%（干重）。然后将半纤维素A和B在121℃和1.03×10⁵Pa下进行水热处理10、30和50分钟。对水热处理后的半纤维素进行了物理化学表征，如克拉森木质素含量和还原糖含量，以研究自水解处理对OPF衍生半纤维素的影响。结果表明，半纤维素A中的克拉森木质素含量高于半纤维素B，且水热处理后降低。水热处理提高了半纤维素的溶解度，这反映了它们较高的还原糖含量。使用高效液相色谱法进行的单糖分析表明，木糖是半纤维素A和B的主要单糖。
