Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan; Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan.
Bioresour Technol. 2015 Jan;176:142-8. doi: 10.1016/j.biortech.2014.11.027. Epub 2014 Nov 15.
The present works investigate hydrothermal pretreatment of oil palm empty fruit bunch and oil palm frond fiber in a batch tube reactor system with temperature and time range from 170 to 250°C and 10 to 20min, respectively. The behavior of soluble sugars, acids, furans, and phenols dramatically changed over treatment severities as determined by HPLC. The cellulose-rich treated solids were analyzed by SEM, WAXD, and BET surface area. Enzymatic hydrolysis was performed from both pretreated slurries and washed solid, and data obtained suggested that tannic acid derived from lignin degradation was a potential cellulase inhibitor. Both partial removal of hemicellulose and migration of lignin during hydrothermal pretreatment caused structural changes on the cellulose-hemicellulose-lignin matrix, resulting in the opening and expansion of specific surface area and pore volume. The current results provided important factors that maximize conversion of cellulose to glucose from oil palm biomass by hydrothermal process.
本研究采用批式管式反应器系统,在 170 至 250°C 的温度范围和 10 至 20 分钟的时间范围内,对油棕空果串和油棕叶片纤维进行了水热预处理。通过高效液相色谱法(HPLC)测定,可溶性糖、酸、呋喃和酚类物质的行为在处理强度下发生了显著变化。用 SEM、WAXD 和 BET 表面积对富含纤维素的处理固体进行了分析。从预处理的浆料和洗涤后的固体进行了酶水解实验,结果表明,来源于木质素降解的单宁酸是潜在的纤维素酶抑制剂。在水热预处理过程中,半纤维素的部分去除和木质素的迁移导致纤维素-半纤维素-木质素基质的结构发生变化,从而导致比表面积和孔体积的增大。本研究结果为通过水热法将油棕生物质中的纤维素最大程度地转化为葡萄糖提供了重要因素。