Umeda F, Yamauchi T, Nakashima N, Ono H, Nawata H, Masuko M, Nakayama K, Tatematsu A
Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Horm Metab Res. 1991 Jun;23(6):274-7. doi: 10.1055/s-2007-1003672.
The effect of glucose on PDGF production and cell proliferation was studied on cultured bovine aortic endothelial cells. PDGF levels were measured using an enzyme-linked immunosorbent assay technique newly developed in our laboratory. The cell proliferation rate was determine on the basis of 3H-thymidine incorporation into cellular DNA. PDGF levels in culture medium were below the detection limit of the assay. However, PDGF levels were measurable in cultured endothelial cells at confluence. Both PDGF production and thymidine incorporation were significantly reduced in the endothelial cells cultured with high concentrations of glucose. These results suggest that reduced PDGF production and cell proliferation may be involved in altered vascular endothelial function in diabetics.
在培养的牛主动脉内皮细胞上研究了葡萄糖对血小板衍生生长因子（PDGF）产生和细胞增殖的影响。使用我们实验室新开发的酶联免疫吸附测定技术测量PDGF水平。根据3H-胸腺嘧啶核苷掺入细胞DNA来确定细胞增殖率。培养基中的PDGF水平低于该测定的检测限。然而，汇合状态下培养的内皮细胞中的PDGF水平是可测量的。在高浓度葡萄糖培养的内皮细胞中，PDGF产生和胸腺嘧啶核苷掺入均显著降低。这些结果表明，PDGF产生减少和细胞增殖可能与糖尿病患者血管内皮功能改变有关。
