Tanaka T, Nishiguchi S, Kuroki T, Kobayashi K, Matsui-Yuasa I, Otani S, Toda T, Monna T, Sukegawa Y
Department of Legal Medicine, Osaka City University Medical School, Japan.
Hepatology. 1991 Oct;14(4 Pt 1):696-700. doi: 10.1016/0270-9139(91)90060-9.
The effects of a single ethanol administration on ornithine decarboxylase induction, polyamine metabolism and DNA synthesis in rat liver after partial hepatectomy were studied. Ethanol given 1 hr before partial hepatectomy at the dose of 2, 3 or 5 gm/kg body wt inhibited the increase in ornithine decarboxylase activity and that in the putrescine level in the liver 4 hr after partial hepatectomy. The hepatectomy increased the amount of ornithine decarboxylase messenger RNA expressed, and this amount was unaffected by ethanol administration. Further, ethanol did not accelerate the degradation of ornithine decarboxylase 4 hr after partial hepatectomy, indicating that the inhibition of ornithine decarboxylase activity caused by ethanol was not caused by a decrease in the ornithine decarboxylase messenger RNA level or by the acceleration of ODC degradation. The single dose of ethanol inhibited [3H]thymidine incorporation into the hepatic DNA 24 hr after partial hepatectomy. The suppression of [3H]thymidine incorporation was partially reversed by the administration of putrescine. These results suggested that ethanol inhibits the increase in ornithine decarboxylase activity after transcription, suppressing the accumulation of putrescine, which prevents DNA synthesis in response to hepatectomy.
研究了单次给予乙醇对大鼠部分肝切除术后肝脏中鸟氨酸脱羧酶诱导、多胺代谢及DNA合成的影响。在部分肝切除术前1小时，以2、3或5克/千克体重的剂量给予乙醇，可抑制部分肝切除术后4小时肝脏中鸟氨酸脱羧酶活性的增加以及腐胺水平的升高。肝切除术增加了鸟氨酸脱羧酶信使RNA的表达量，而给予乙醇对此量无影响。此外，部分肝切除术后4小时，乙醇并未加速鸟氨酸脱羧酶的降解，这表明乙醇对鸟氨酸脱羧酶活性的抑制并非由鸟氨酸脱羧酶信使RNA水平的降低或鸟氨酸脱羧酶降解的加速所致。单次给予乙醇可抑制部分肝切除术后24小时[3H]胸腺嘧啶核苷掺入肝脏DNA。给予腐胺可部分逆转[3H]胸腺嘧啶核苷掺入的抑制作用。这些结果表明，乙醇在转录后抑制鸟氨酸脱羧酶活性的增加，抑制腐胺的积累，而腐胺的积累可阻止因肝切除术而引起的DNA合成。
