30只大熊猫粪便细菌中抗生素耐药性的表型和基因型特征分析
Phenotypic and genotypic characterisation of antimicrobial resistance in faecal bacteria from 30 Giant pandas.
作者信息
Zhang An-Yun, Wang Hong-Ning, Tian Guo-Bao, Zhang Yi, Yang Xin, Xia Qing-Qing, Tang Jun-Ni, Zou Li-Kou
机构信息
Bioengineering Research Center for Animal Disease Prevention and Control, School of Life Science, Sichuan University, Chengdu 610064, China.
出版信息
Int J Antimicrob Agents. 2009 May;33(5):456-60. doi: 10.1016/j.ijantimicag.2008.10.030. Epub 2009 Jan 24.
To study the prevalence of antimicrobial resistance in faecal bacteria from Giant pandas in China, 59 isolates were recovered from faecal pats of 30 Giant pandas. Antimicrobial susceptibility testing of the isolates was performed by the standardised disk diffusion method (Kirby-Bauer). Of the 59 study isolates, 32.20% were resistant to at least one antimicrobial and 16.95% showed multidrug-resistant phenotypes. Thirteen drug resistance genes [aph(3')-IIa, aac(6')-Ib, ant(3'')-Ia, aac(3)-IIa, sul1, sul2, sul3, tetA, tetC, tetM, cat1, floR and cmlA] were analysed using four primer sets by multiplex polymerase chain reaction (PCR). The detection frequency of the aph(3')-IIa gene was the highest (10.17%), followed by cmlA (8.47%). The genes aac(6')-Ib, sul2 and tetA were not detected. PCR products were confirmed by DNA sequence analysis. The results revealed that multidrug resistance was widely present in bacteria isolated from Giant pandas.
为研究中国大熊猫粪便细菌中的抗菌药物耐药性流行情况，从30只大熊猫的粪便样本中分离出59株菌株。采用标准化纸片扩散法（Kirby-Bauer法）对分离菌株进行药敏试验。在59株研究菌株中，32.20%对至少一种抗菌药物耐药，16.95%表现出多重耐药表型。使用四组引物通过多重聚合酶链反应（PCR）分析了13种耐药基因[aph(3')-IIa、aac(6')-Ib、ant(3'')-Ia、aac(3)-IIa、sul1、sul2、sul3、tetA、tetC、tetM、cat1、floR和cmlA]。aph(3')-IIa基因的检测频率最高（10.17%），其次是cmlA（8.47%）。未检测到aac(6')-Ib、sul2和tetA基因。通过DNA序列分析对PCR产物进行了确认。结果显示，多重耐药性在从大熊猫分离出的细菌中广泛存在。
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