Carbone M C, Pinto M, Antonelli F, Amicarelli F, Balata M, Belli M, Conti Devirgiliis L, Ioannucci L, Nisi S, Sapora O, Satta L, Simone Giustina, Sorrentino E, Tabocchini M A
Museo Storico della Fisica e Centro Studi e Ricerche "Enrico Fermi", Rome, Italy.
Radiat Environ Biophys. 2009 Apr;48(2):189-96. doi: 10.1007/s00411-008-0208-6. Epub 2009 Jan 24.
Previously we reported that yeast and Chinese hamster V79 cells cultured under reduced levels of background environmental ionizing radiation show enhanced susceptibility to damage caused by acute doses of genotoxic agents. Reduction of environmental radiation dose rate was achieved by setting up an underground laboratory at Laboratori Nazionali del Gran Sasso, central Italy. We now report on the extension of our studies to a human cell line. Human lymphoblastoid TK6 cells were maintained under identical in vitro culture conditions for six continuous months, at different environmental ionizing radiation levels. Compared to "reference" environmental radiation conditions, we found that cells cultured in the underground laboratories were more sensitive to acute exposures to radiation, as measured both at the level of DNA damage and oxidative metabolism. Our results are compatible with the hypothesis that ultra-low dose rate ionizing radiation, i.e. environmental radiation, may act as a conditioning agent in the radiation-induced adaptive response.
此前我们报道，在背景环境电离辐射水平降低的条件下培养的酵母和中国仓鼠V79细胞，对急性剂量的遗传毒性剂造成的损伤表现出更高的敏感性。通过在意大利中部大萨索山国家实验室建立一个地下实验室，实现了环境辐射剂量率的降低。我们现在报告将研究扩展到人类细胞系的情况。人类淋巴母细胞TK6细胞在不同的环境电离辐射水平下，于相同的体外培养条件下连续维持六个月。与“参考”环境辐射条件相比，我们发现，在地下实验室中培养的细胞对急性辐射暴露更敏感，这在DNA损伤和氧化代谢水平上均有体现。我们的结果与以下假设相符：即超低剂量率电离辐射，也就是环境辐射，可能在辐射诱导的适应性反应中充当一种预处理剂。
