心房颤动患者心房组织中核因子-κB 的激活和肿瘤坏死因子-α的增加。
Activated nuclear factor-kappaB and increased tumor necrosis factor-alpha in atrial tissue of atrial fibrillation.
机构信息
Department of Cardiology, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, China.
出版信息
Scand Cardiovasc J. 2009;43(5):292-7. doi: 10.1080/14017430802651803.
OBJECTIVES
To depict the interaction between atrial fibrillation (AF) and inflammatory reaction, studies were taken to measure the activity of NF-kappaB in myocardium, the concentration of regional inflammatory factors and the pathological process of the right atrium in patients with AF.
DESIGN
Patients with valvular disease with AF or sinus rhythm (SR) were recruited and compared. Before the extracorporal circulation, about 250 mg tissue of right atrium was collected for pathological examination. The activity of NF-kappaB in myocardium was measured by electrophoretic mobility shift assay (EMSA), and the concentration of cardiac tissue interleukin-6 (IL-6) and tumor necrosis factor (TNF-alpha) was determined by radioimmuoassay.
RESULTS
Patients with valvular disease with AF exhibited higher NF-kappaB activity, higher concentration of TNF-alpha and IL-6, severe lymphomonocyte infiltration, and more fibrosis than those patients with valvular disease with SR. There were significant positive correlations among NF-kappaB activity and levels of TNF-alpha and IL-6 and collagen volume fraction.
CONCLUSIONS
This study proved the presence of inflammation in atrial myocardium by triggering inflammatory reaction.
目的
描绘心房颤动（AF）与炎症反应之间的相互作用，研究测量 NF-κB 在心肌中的活性、局部炎症因子的浓度以及 AF 患者右心房的病理过程。
设计
招募并比较瓣膜病合并 AF 或窦性节律（SR）的患者。在体外循环前，采集约 250mg 右心房组织进行病理检查。通过电泳迁移率变动分析（EMSA）测量心肌 NF-κB 的活性，并通过放射免疫法测定心脏组织白细胞介素-6（IL-6）和肿瘤坏死因子（TNF-α）的浓度。
结果
与瓣膜病合并 SR 的患者相比，瓣膜病合并 AF 的患者 NF-κB 活性更高、TNF-α 和 IL-6 浓度更高、淋巴细胞单核细胞浸润更严重、纤维化程度更严重。NF-κB 活性与 TNF-α 和 IL-6 水平以及胶原容积分数之间存在显著的正相关。
结论
本研究通过触发炎症反应证明了心房心肌中的炎症存在。
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