Gilmore Christopher P, DeLuca Gabriele C, Bö Lars, Owens Trudy, Lowe James, Esiri Margaret M, Evangelou Nikos
Department of Neurology, Queens Medical Centre NHS Trust, Nottingham.
Brain Pathol. 2009 Oct;19(4):642-9. doi: 10.1111/j.1750-3639.2008.00228.x. Epub 2008 Dec 19.
The objective of this study was to assess neuronal pathology in the spinal cord in multiple sclerosis (MS), both within myelinated and demyelinated tissue. Autopsy material was obtained from 38 MS cases and 21 controls. Transverse sections were taken from three spinal cord levels and stained using Luxol Fast Blue/Cresyl Violet and myelin protein immunohistochemistry. Measurements of neuronal number and size were made for all neurons within the anterior horns of the gray matter. Neurons were classified as motoneurons or interneurons according to size criteria. In comparison with controls, both motoneuron and interneuron number were reduced in MS cases at the upper cervical (interneuron P = 0.0549; motoneuron P = 0.0073) and upper thoracic (interneuron P = 0.0507; motoneuron P = 0.0144), but not the lumbar level. Interneuron cross-sectional area was reduced in MS cases at all levels (upper cervical, P = 0.0000; upper thoracic, P = 0.0002; lumbar, P = 0.0337). Neuronal loss appears to be predominantly related to local gray matter plaques, whereas interneuron atrophy occurs in both myelinated and demyelinated areas.
本研究的目的是评估多发性硬化症（MS）患者脊髓中髓鞘化和脱髓鞘组织内的神经元病理学。尸检材料取自38例MS患者和21例对照。从脊髓的三个节段获取横切面，并用Luxol固蓝/甲酚紫和髓磷脂蛋白免疫组织化学进行染色。对灰质前角内的所有神经元进行神经元数量和大小的测量。根据大小标准将神经元分为运动神经元或中间神经元。与对照组相比，MS患者在上颈段（中间神经元P = 0.0549；运动神经元P = 0.0073）和上胸段（中间神经元P = 0.0507；运动神经元P = 0.0144）的运动神经元和中间神经元数量均减少，但腰段未减少。MS患者各节段的中间神经元横截面积均减小（上颈段，P = 0.0000；上胸段，P = 0.0002；腰段，P = 0.0337）。神经元丢失似乎主要与局部灰质斑块有关，而中间神经元萎缩则发生在髓鞘化和脱髓鞘区域。
