Soulieres D, Rousseau A, Deschenes J, Tremblay M, Tardif M, Pelletier G
Département d'Ophtalmologie, l'Université Laval, Sainte-Foy, Québec, Canada.
Int J Cancer. 1991 Oct 21;49(4):498-503. doi: 10.1002/ijc.2910490404.
We have evaluated the ganglioside composition of 20 primary uveal melanomas, of 2 cell lines derived from 2 uveal melanomas, of a liver metastasis from an uveal melanoma, and of 8 normal choroids. The results show that normal choroid tissue has a ganglioside content similar to the primary tumors of uveal melanoma except for GD1a, GD1b, and GT1b, which are present only on normal choroid tissues. On the other hand, the uveal melanomas have similarities with cutaneous melanomas, since GM3 (74%) and GD3 (25%) are found in both tissues and are present in about the same amounts. However, GM1 was found in 50%, GM2 in 20% and GD2 in none of the uveal melanomas. According to data published by others, cutaneous melanoma biopsies have no GM1, whereas GM2 is present in 100% and GD2 in 71% of tumor tissues. Transplantation of the 2 cell lines subcutaneously into nude mice resulted in the growth of tumors which had a ganglioside profile larger than that of the primary tumors. GM3 was significantly diminished and GD3 significantly increased in the primary uveal melanoma from patients who had received radiotherapy before enucleation compared with those who did not have radiotherapy. These results show that uveal melanomas contain gangliosides that could be used as targets for monoclonal antibody therapy.
我们评估了20例原发性葡萄膜黑色素瘤、源自2例葡萄膜黑色素瘤的2个细胞系、1例葡萄膜黑色素瘤肝转移灶以及8例正常脉络膜的神经节苷脂组成。结果显示，正常脉络膜组织的神经节苷脂含量与葡萄膜黑色素瘤的原发性肿瘤相似，但GD1a、GD1b和GT1b仅存在于正常脉络膜组织中。另一方面，葡萄膜黑色素瘤与皮肤黑色素瘤有相似之处，因为在这两种组织中均发现了GM3（74%）和GD3（25%），且含量大致相同。然而，在葡萄膜黑色素瘤中，50%发现有GM1，20%发现有GM2，未发现有GD2。根据其他人发表的数据，皮肤黑色素瘤活检标本中没有GM1，而在100%的肿瘤组织中存在GM2，71%存在GD2。将这2个细胞系皮下移植到裸鼠体内导致肿瘤生长，其神经节苷脂谱比原发性肿瘤更大。与未接受放疗的患者相比，在眼球摘除术前接受放疗的患者的原发性葡萄膜黑色素瘤中，GM3显著减少，GD3显著增加。这些结果表明，葡萄膜黑色素瘤含有可作为单克隆抗体治疗靶点的神经节苷脂。
