基于SCP B基因的聚合酶链反应对B族链球菌进行母婴筛查：一项初步研究。

Maternal and neonatal screening for Group B streptococci by SCP B gene based PCR: a preliminary study.

作者信息

Elbaradie Sahar M Y, Mahmoud Manal, Farid Mona

机构信息

Department of Obstetrics and Gynecology, AlFayom University, Egypt.

出版信息

Indian J Med Microbiol. 2009 Jan-Mar;27(1):17-21.

PMID:19172053

Abstract

OBJECTIVE

To detect the magnitude of group B streptococcal (GBS) colonization and disease among a sample of pregnant women and their infants in Egypt.

STUDY DESIGN

Prospective observational study.

PARTICIPANTS

The study included 95 pregnant females, 35-37 weeks of gestational age, attending the antenatal outpatient clinic at AlFayom University Hospital between September 2006 and June 2007. All participants were screened with vaginorectal swabs by a conventional GBS PCR assay. Participants were grouped into group A (GBS present, 17 patients) and group B (GBS absent, 78 patients). Details with regard to labor and delivery were recorded and placental pathology was examined to detect histological chorioamnionitis. Ninety-five infant data were also recorded. All neonates of group A (17 out of 95 with known positive maternal GBS) underwent collection of simultaneous specimens from surface sites for PCR before their first bath and within four hours of birth.

RESULTS

GBS carriage rate in the study sample was 17.89%. Chorioamnionitis confirmed in three patients by placental pathology (one was in group A and two in group B) was statistically not significant. Twenty-two women had rupture of membranes (< 12 hours) before delivery (four from group A and 18 from group B) that was not statistically significant. There were three infants out of 17 in group A who had GBS colonized at one or more sites by PCR which was statistically significant. However, only one infant was admitted to neonatal intensive care unit (NICU) that was not statistically significant.

CONCLUSION

Maternal GBS carriage is associated with a significant increase in neonatal infection rate but is not associated with an increase in neonatal intensive care admission. An accurate evaluation of colonization rate (using a larger sample) is desired to evaluate neonatal invasive disease and determine the cost effectiveness of PCR to select an appropriate preventive strategy in Egypt.

摘要

目的

检测埃及孕妇及其婴儿样本中B族链球菌（GBS）的定植率和发病率。

研究设计

前瞻性观察性研究。

参与者

该研究纳入了95名孕龄为35 - 37周的孕妇，她们于2006年9月至2007年6月期间在法尤姆大学医院产前门诊就诊。所有参与者均通过传统GBS聚合酶链反应（PCR）检测法进行阴道直肠拭子筛查。参与者被分为A组（GBS阳性，17例患者）和B组（GBS阴性，78例患者）。记录分娩和产程的详细情况，并检查胎盘病理以检测组织学绒毛膜羊膜炎。还记录了95例婴儿的数据。A组的所有新生儿（95例中有17例母亲GBS已知阳性）在首次沐浴前及出生后4小时内从体表部位采集同步标本进行PCR检测。

结果

研究样本中GBS携带率为17.89%。通过胎盘病理确诊的3例绒毛膜羊膜炎患者（1例在A组，2例在B组），差异无统计学意义。22名女性在分娩前胎膜破裂（< 12小时）（4例来自A组，18例来自B组），差异无统计学意义。A组17例中有3例婴儿通过PCR检测在一个或多个部位有GBS定植，差异有统计学意义。然而，只有1例婴儿入住新生儿重症监护病房（NICU），差异无统计学意义。