一种用于测定血清中酸性磷酸酶活性的自动化连续监测程序。
An automated continuous-monitoring procedure for the determination of acid phosphatase activity in serum.
作者信息
Warren R J, Moss D W
出版信息
Clin Chim Acta. 1977 Jun 1;77(2):179-88. doi: 10.1016/0009-8981(77)90025-0.
PMID:19173
Abstract
The method of Hillmann, in which hydrolysis of alpha-naphthyl phosphate by acid phosphatase is coupled to the formation of an alpha-naphthol-Fast Red TR azo-compound, has been adapted for use with the LKB Produkter AB 8600 reaction rate analyzer. Factors which affect the reproducibility of the method are described and its performance is shown to be superior to that of a manual phenyl-phosphate procedure.
摘要
希尔曼方法是将酸性磷酸酶对α-萘基磷酸酯的水解与α-萘酚-固红TR偶氮化合物的形成相耦合，现已适用于LKB Produkter AB 8600反应速率分析仪。文中描述了影响该方法重现性的因素，并表明其性能优于手动苯基磷酸酯法。
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