School of Polymers and High Performance Materials, Shelby F. Thames Polymer Science Research Center, The University of Southern Mississippi, Hattiesburg, Mississippi 39406, USA.
Biomacromolecules. 2009 Mar 9;10(3):623-9. doi: 10.1021/bm8013473.
Surface reactions were performed on polypropylene (PP) surfaces to retard the simultaneous growth of Staphylococcus aureus (S. aureus) and Pseudomonas putida (P. putida) bacteria. Microwave plasma reactions in the presence of maleic anhydride (MA) resulted in the formation of acid groups on the surface of PP. Such surfaces were further modified by conducting two parallel reactions: (1) poly(ethylene glycol) (PEG) was attached to COOH groups of the PP surface, followed by penicillin V (PEN) reactions to target S. aureus destruction and (2) diglycidyl PEG was attached, followed by gentamicin (GEN) reactions, to create antimicrobial surfaces targeted at P. putida . Simultaneous gram "+" and gram "-" resistance was obtained by varying the PEN/GEN ratios on such modified PP surfaces, thus providing the controllable degree of gram "+" and gram "-" antimicrobial strength. While spectroscopic analyses revealed chemical attachments of PEN and GEN, the effectiveness against proliferation of S. aureus (Gram +) and P. putida (Gram -) bacteria was determined using liquid culture tests. These studies show for the first time the formation of tunable antimicrobial polypropylene surfaces with controllable strength.
对聚丙烯 (PP) 表面进行了表面反应，以阻止金黄色葡萄球菌 (S. aureus) 和铜绿假单胞菌 (P. putida) 细菌的同时生长。在马来酸酐 (MA) 的存在下进行微波等离子体反应，导致 PP 表面形成酸基团。通过进行两个平行反应进一步修饰这些表面：(1) 将聚乙二醇 (PEG) 连接到 PP 表面的 COOH 基团上，然后进行青霉素 V (PEN) 反应以靶向 S. aureus 的破坏，以及 (2) 连接二缩水甘油基聚乙二醇，然后进行庆大霉素 (GEN) 反应，以创建针对 P. putida 的抗菌表面。通过改变修饰后的 PP 表面上 PEN/GEN 的比例，可以获得同时针对革兰氏阳性菌和革兰氏阴性菌的抗性，从而提供了可控制的革兰氏阳性菌和革兰氏阴性菌抗菌强度。虽然光谱分析显示 PEN 和 GEN 的化学连接，但使用液体培养试验确定了它们对金黄色葡萄球菌（革兰氏阳性菌）和铜绿假单胞菌（革兰氏阴性菌）细菌增殖的有效性。这些研究首次展示了具有可控强度的可调抗菌聚丙烯表面的形成。
