Bennett Mark J, Steiner Robert R
Department of Forensic Science, Virginia Commonwealth University, Richmond, VA 23284, USA.
J Forensic Sci. 2009 Mar;54(2):370-5. doi: 10.1111/j.1556-4029.2008.00955.x. Epub 2009 Jan 27.
A new screening method for detecting gamma-hydroxybutyric acid (GHB) in drink matrices, using the IonSense, Inc. (Saugus, MA) direct analysis in real time (DART) ion source coupled to a JEOL exact mass time-of-flight mass spectrometer (AccuTOF), was validated and compared with the current screening methodology. The DART ion source allows for analysis of samples under ambient conditions with little to no sample preparation. Fifty drink specimens were spiked at levels of 1, 2, 3, and 4 mg/mL GHB, and analyzed on the AccuTOF-DART. Positive detection of GHB occurred for each of the samples at each concentration level, giving 100% accuracy for the samples tested. Twenty-five of the 50 drink specimens were spiked at 1 mg/mL GHB and tested using a color test known as the GHB Color Test #3. Only two of these 25 specimens tested positive for the presence of GHB, giving only 8% accuracy. Implementation of this new methodology as a screening tool for GHB analysis will quickly eliminate negative specimens allowing the examiner to focus analysis time on those that screened positive.
一种用于检测饮料基质中γ-羟基丁酸（GHB）的新型筛查方法得到了验证，并与当前的筛查方法进行了比较。该方法使用IonSense公司（马萨诸塞州索格斯）的实时直接分析（DART）离子源与JEOL高分辨飞行时间质谱仪（AccuTOF）联用。DART离子源能够在几乎无需样品制备的环境条件下对样品进行分析。五十份饮料样本分别添加了浓度为1、2、3和4 mg/mL的GHB，并在AccuTOF-DART上进行分析。每个浓度水平的所有样品均检测出GHB呈阳性，测试样品的准确率达到100%。五十份饮料样本中有二十五份添加了1 mg/mL的GHB，并使用一种名为GHB Color Test #3的颜色测试进行检测。这二十五份样本中只有两份检测出GHB呈阳性，准确率仅为8%。将这种新方法用作GHB分析的筛查工具，将能够快速排除阴性样本，使检测人员能够将分析时间集中在筛查呈阳性的样本上。
