Kricka L J, Carter T J
Clin Chim Acta. 1977 Aug 15;79(1):141-7. doi: 10.1016/0009-8981(77)90471-5.
The limited stability of immobilised enzymes is a major factor restricting their use in clinical chemistry. The immobilisation procedure described here affords a simple method of enhancing the stability of certain enzymes immobilised on nylon. Studies with alcohol dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase have indicated that a significant increase in stability can be obtained using this immobilisation procedure as compared with conventional procedures. As anticipated the immobilisation procedure was unsatisfactory for oxidases such as glucose oxidase.
固定化酶的稳定性有限是限制其在临床化学中应用的主要因素。本文所述的固定化方法提供了一种增强某些固定在尼龙上的酶稳定性的简单方法。对乙醇脱氢酶和3-磷酸甘油醛脱氢酶的研究表明，与传统方法相比，使用这种固定化方法可显著提高稳定性。正如预期的那样，该固定化方法对葡萄糖氧化酶等氧化酶并不理想。
