Thompson R Q, Kim H
Department of Chemistry, Oberlin College, OH 44074.
Biochim Biophys Acta. 1988 Jan 12;964(1):116-20. doi: 10.1016/0304-4165(88)90075-x.
Equations describing the flow kinetics of immobilized glucose oxidase in open nylon tubes and in tubes filled with solid glass spheres were experimentally determined. Reactors of three different tube (dt) and bead (db) diameters were tested using various linear flow rates (vf) and glucose concentrations ([S]). The kinetics of the open-tubular reactors were described by [P] = 1.5.10(-3) [S] L0.86 vf-0.78 dt-0.9 and the kinetics of the beaded-tubular reactors by [P] = 5.10(-3) [S] L0.98 vf-0.75 dt-1.0 (db/dt)2.7, where [P] equals the concentration of H2O2 formed. The aspect ratio, db/dt, is the critical design factor for beaded reactors.
通过实验确定了描述固定化葡萄糖氧化酶在开放尼龙管和填充固体玻璃珠的管中的流动动力学的方程。使用三种不同的管径（dt）和珠径（db）的反应器，在各种线性流速（vf）和葡萄糖浓度（[S]）下进行了测试。开放管式反应器的动力学由[P]=1.5×10⁻³ [S] L⁰.⁸⁶ vf⁻⁰.⁷⁸ dt⁻⁰.⁹描述，珠状管式反应器的动力学由[P]=5×10⁻³ [S] L⁰.⁹⁸ vf⁻⁰.⁷⁵ dt⁻¹.⁰ (db/dt)².⁷描述，其中[P]等于生成的H₂O₂的浓度。长径比db/dt是珠状反应器的关键设计因素。
