Badet Cécile, Furiga Aurélie, Thébaud Noélie
Laboratoire de Microbiologie, UFR d'Odontologie, Université Victor Ségalen Bordeaux 2, 33082 Bordeaux cedex, France.
Oral Health Prev Dent. 2008;6(4):337-41.
The aim of the present study was to examine whether xylitol, at different concentrations, inhibits the formation of an experimental model of oral biofilm.
Biofilms of six bacterial species (Streptococcus mutans, Streptococcus sobrinus, Lactobacillus rhamnosus, Actinomyces viscosus, Porphyromonas gingivalis and Fusobacterium nucleatum) were prepared on hydroxyapatite (HA) discs according to the Zürich Biofilm Model. Xylitol was tested at two concentrations, 1% and 3%. At the end of their designated incubation times, some HA discs were destined for confocal laser scanning microscopy (CLSM) and the others were harvested using a sterile surgical instrument. Aliquots of harvested biofilms were diluted and plated onto specific media. After a 48-h anaerobic incubation at 37 degrees C, the colony-forming units (CFUs) were counted.
CLSM images showed that only a small amount of isolated bacteria was observed on the surface of HA discs. Culture of harvested biofilms showed an inhibition in the growth of different species included in the biofilms.
Xylitol has a clear inhibitory effect on the formation of the experimental biofilms. This study shows that xylitol is not only efficient in inhibiting the acid production of cariogenic bacteria, but also in preventing the formation of a multispecies biofilm; it confirms the relevance of the use of this polyol for the prevention of oral diseases caused by dental plaque.
本研究旨在探讨不同浓度的木糖醇是否能抑制口腔生物膜实验模型的形成。
根据苏黎世生物膜模型，在羟基磷灰石（HA）圆盘上制备六种细菌（变形链球菌、远缘链球菌、鼠李糖乳杆菌、粘性放线菌、牙龈卟啉单胞菌和具核梭杆菌）的生物膜。测试了1%和3%两种浓度的木糖醇。在指定的孵育时间结束时，一些HA圆盘用于共聚焦激光扫描显微镜（CLSM）检查，另一些则使用无菌手术器械采集。采集的生物膜等分试样进行稀释，然后接种到特定培养基上。在37℃厌氧培养48小时后，计算菌落形成单位（CFU）。
CLSM图像显示，在HA圆盘表面仅观察到少量分离的细菌。采集的生物膜培养显示，生物膜中包含的不同菌种的生长受到抑制。
木糖醇对实验性生物膜的形成具有明显的抑制作用。本研究表明，木糖醇不仅能有效抑制致龋菌的产酸，还能防止多菌种生物膜的形成；这证实了使用这种多元醇预防牙菌斑引起的口腔疾病的相关性。
