Krishna Murthy M N, Rajalakshmi S, Satyabodha J A, Nagaraja K V
Central Food Technological Research Institute, Mysore, India.
J Assoc Off Anal Chem. 1991 Jul-Aug;74(4):698-9.
A simple and precise method of detecting brominated vegetable oil (BVO) in soft drinks is described. After extraction of BVO using diethyl ether, the concentrated ethereal solution was treated with a small quantity of zinc dust to convert the organic bromide to inorganic form; the solution was subsequently treated with lead dioxide to liberate bromine. The bromine evolved was detected by means of fluorescein-impregnated filter paper strip that turns pink because eosin is formed. The test can detect as low as 10 ppm (2 mg/200 ml) of BVO under experimental conditions. Gas chromatography was carried out on sodium methoxide derivatives prepared from ether extract for quantitation.
描述了一种简单而精确的检测软饮料中溴化植物油（BVO）的方法。用乙醚萃取BVO后，将浓缩的乙醚溶液用少量锌粉处理，使有机溴转化为无机形式；随后该溶液用二氧化铅处理以释放出溴。所释放出的溴通过浸渍有荧光素的滤纸条进行检测，滤纸条会因形成曙红而变成粉红色。在实验条件下，该测试可检测低至10 ppm（2毫克/200毫升）的BVO。对由乙醚提取物制备的甲醇钠衍生物进行气相色谱分析以进行定量。
