Windsor Martin L, Eisenberg Mark, Gordon-Thomson Clare, Moore Geoffrey P M
Research and Development, Sylvan Scientific Ltd, New South Wales, Australia.
Australas J Dermatol. 2009 Feb;50(1):29-35. doi: 10.1111/j.1440-0960.2008.00512.x.
Studies of skin graft behaviour in rodent excisional wound models are limited by the dominance of wound contracture and graft sloughing as primary healing responses. To slow skin contraction, polytetrafluoroethylene (Teflon) rings were inserted into dorso-lateral full-thickness wounds in SCID mice. Cultured skin substitutes (OrCel), composed of cultured human keratinocytes and fibroblasts in a bovine collagen sponge, were implanted within the rings. Examination and histology of grafts 14 days later showed graft take in four of six recipients, with 90% epithelialization and wound contraction of 31-47%. Immunohistochemical studies, using human-specific antisera to distinguish graft from host tissues, showed that regenerated tissue was predominantly human. Staining with anticytokeratin, revealed a multilayered, stratified neoepidermis. HBG were identified in keratinocytes in all epidermal layers. Langerhans cells were absent. Antihuman vimentin, used as a fibroblast marker, confirmed that cells of the neodermis were primarily of human origin. Neoepidermal keratinocytes, primarily in the basal and suprabasal layers, were also stained. Results suggest that the poly(tetrafluoroethylene) ring inhibited graft sloughing and provided a more favourable environment for the skin substitute to regenerate a substantially normal human skin.
在啮齿动物切除伤口模型中，皮肤移植行为的研究受到伤口挛缩和移植皮片脱落这两种主要愈合反应的限制。为减缓皮肤收缩，将聚四氟乙烯（特氟龙）环插入SCID小鼠的背外侧全层伤口中。将由培养的人角质形成细胞和成纤维细胞置于牛胶原海绵中组成的培养皮肤替代物（OrCel）植入环内。14天后对移植物进行检查和组织学分析，结果显示6只受体中有4只出现移植物存活，上皮化率达90%，伤口收缩率为31% - 47%。使用人特异性抗血清区分移植物和宿主组织的免疫组织化学研究表明，再生组织主要为人类组织。用抗细胞角蛋白染色显示出多层分层的新表皮。在所有表皮层的角质形成细胞中均鉴定出人类β - 珠蛋白（HBG）。未发现朗格汉斯细胞。用作成纤维细胞标志物的抗人波形蛋白证实，新真皮细胞主要来源于人类。新表皮角质形成细胞，主要位于基底层和基底上层，也呈阳性染色。结果表明，聚四氟乙烯环抑制了移植皮片的脱落，并为皮肤替代物再生出基本正常的人类皮肤提供了更有利的环境。
