ilvGMEDA操纵子内部ilvEp启动子处支链氨基酸的缺失与生长速率调控
The absence of branched-chain amino acid and growth rate control at the internal ilvEp promoter of the ilvGMEDA operon.
作者信息
Harms E H, Umbarger H E
机构信息
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
出版信息
J Bacteriol. 1991 Oct;173(20):6446-52. doi: 10.1128/jb.173.20.6446-6452.1991.
Abstract
The question of whether the promoter ilvEp, located in the coding region of ilvM, the second structural gene in the ilvGMEDA operon, is subject to either amino acid- or growth rate-mediated regulation is examined. The experiments described here were performed with ilvEp-cat and ilvEp-lac fusions carried as single copies on the chromosome. The activity of the ilvEp promoter was found to respond neither to the availability of branched-chain amino acids nor to a wide range of growth rates between 35 to 390 min. In the absence of any known role for the products of the ilvGMEDA operon when repressing levels of branched-chain amino acids are present, there appears to be only a gratuitous role for the transcription at ilvEp.
摘要
位于ilvGMEDA操纵子第二个结构基因ilvM编码区域的启动子ilvEp是否受氨基酸或生长速率介导的调控这一问题得到了研究。这里所描述的实验是使用在染色体上以单拷贝形式存在的ilvEp-cat和ilvEp-lac融合体进行的。结果发现，ilvEp启动子的活性既不响应支链氨基酸的可用性，也不响应35至390分钟之间的广泛生长速率。当存在支链氨基酸的阻遏水平时，在ilvGMEDA操纵子产物不存在任何已知作用的情况下，ilvEp处的转录似乎仅起无端作用。
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