1型和2型猪圆环病毒的复制蛋白与衣壳蛋白和宿主蛋白的相互作用。
Interaction of the replication proteins and the capsid protein of porcine circovirus type 1 and 2 with host proteins.
作者信息
Finsterbusch Tim, Steinfeldt Tobias, Doberstein Kai, Rödner Claudia, Mankertz Annette
机构信息
Division of Viral Infections (FG12), Robert Koch-Institute, Nordufer 20, 13353 Berlin, Germany.
出版信息
Virology. 2009 Mar 30;386(1):122-31. doi: 10.1016/j.virol.2008.12.039.
Porcine circovirus type 2 (PCV2) is an important pathogen in swine, whereas porcine circovirus type 1 (PCV1) is apathogenic. To analyze the interactions between PCV and its host, we have used a yeast two-hybrid assay to identify cellular proteins interacting with Cap and Rep proteins of both PCV genotypes. Six cellular proteins were found to interact with Cap (MKRN1, gC1qR, Par-4, NAP1, NPM1 and Hsp40) and three with Rep (ZNF265, TDG and VG5Q). These interactions were confirmed by co-immunoprecipitation. Investigation of the localisation of the proteins by immunofluorescence revealed in some cases only limited spatial overlapping with Cap, while in others a clear co-localisation and prominent protein redistribution was observed. The nine cellular proteins are associated with distinct aspects of viral lifecycle and our data is likely to support future research in the field of PCV2 pathogenesis.
猪圆环病毒2型（PCV2）是猪的一种重要病原体，而猪圆环病毒1型（PCV1）无致病性。为了分析PCV与其宿主之间的相互作用，我们利用酵母双杂交试验来鉴定与两种PCV基因型的Cap和Rep蛋白相互作用的细胞蛋白。发现六种细胞蛋白与Cap相互作用（MKRN1、gC1qR、Par-4、NAP1、NPM1和Hsp40），三种与Rep相互作用（ZNF265、TDG和VG5Q）。这些相互作用通过免疫共沉淀得到证实。通过免疫荧光对蛋白定位的研究表明，在某些情况下，与Cap的空间重叠有限，而在其他情况下，则观察到明显的共定位和显著的蛋白重新分布。这九种细胞蛋白与病毒生命周期的不同方面相关，我们的数据可能会支持PCV2发病机制领域的未来研究。
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