Taguchi Meiko, Chida Kohsuke
Department of Anatomy, School of Allied Health Sciences, Kitasato University, Sagamihara, Kanagawa 228-8555, Japan.
Acta Histochem Cytochem. 2008 Dec 27;41(6):149-55. doi: 10.1267/ahc.08009. Epub 2008 Oct 28.
We investigated the changes caused by microtubule disruption in cell contact-induced translocation of alkaline phosphatase (ALP) from the Golgi area to the plasma membrane in McA-RH 7777 cells. When the cells were treated with colchicine, the tubular structure of microtubules in the cytoplasm was lost. Colchicine treatment also resulted in the appearance of numerous dots containing mannosidase II (man II) throughout the cytoplasm. Moreover, ALP was distributed in small dots throughout the cytoplasm, as well as in all regions of the plasma membrane, although it was most concentrated at sites of intercellular contact. On the other hand, when the cells were incubated in basal medium after colchicine treatment, large spots containing ALP reappeared in the perinuclear cytoplasm more quickly than the accumulation of small dots containing man II. These findings suggest that colchicine causes disassembly of the Golgi complex into fragments, which scatter throughout the cytoplasm, but that it does not interfere with translocation of ALP to the plasma membrane. Furthermore, cytoplasmic ALP may be localized at sites other than the Golgi complex.
我们研究了微管破坏对McA-RH 7777细胞中细胞接触诱导碱性磷酸酶（ALP）从高尔基体区域向质膜转位所造成的变化。当用秋水仙碱处理细胞时，细胞质中微管的管状结构消失。秋水仙碱处理还导致整个细胞质中出现大量含有甘露糖苷酶II（man II）的小点。此外，碱性磷酸酶分布于整个细胞质中的小点以及质膜的所有区域，尽管它在细胞间接触部位最为集中。另一方面，当秋水仙碱处理后的细胞在基础培养基中孵育时，含有碱性磷酸酶的大斑点在核周细胞质中重新出现的速度比含有甘露糖苷酶II的小点积累的速度更快。这些发现表明，秋水仙碱导致高尔基体复合体分解成碎片，这些碎片散布于整个细胞质中，但它并不干扰碱性磷酸酶向质膜的转位。此外，细胞质碱性磷酸酶可能定位于高尔基体复合体以外的其他部位。
