Chida K
Department of Pathology, Kitasato University School of Hygienic Sciences, Kanagawa, Japan.
Okajimas Folia Anat Jpn. 1993 Dec;70(5):203-7. doi: 10.2535/ofaj1936.70.5_203.
The author attempted the detection of alkaline phosphatase (ALP) protein in various rat organs, which were fixed with formalin and embedded in paraffin, by means of the avidin-biotin peroxidase complex (ABC) method. Reaction products were detected after a usual incubation time of 30 min at room temperature in anti-ALP antibody. Positive reactions were observed in the kidney and liver but not in the small intestine, and this indicates that the antibody used in the present study reacts only with the tissue-nonspecific type of ALP.
作者尝试通过抗生物素蛋白-生物素过氧化物酶复合物（ABC）法，检测用福尔马林固定并包埋于石蜡中的各种大鼠器官中的碱性磷酸酶（ALP）蛋白。在室温下用抗ALP抗体常规孵育30分钟后检测反应产物。在肾脏和肝脏中观察到阳性反应，而在小肠中未观察到，这表明本研究中使用的抗体仅与组织非特异性类型的ALP发生反应。
