Karelin A A, Mardashev S R
Probl Endokrinol (Mosk). 1977 Jan-Feb;23(1):79-83.
A study was made of the influence of human chorionic gonadotropin (HCGT) on the activity of guanidineacetate-N-methyltranspherase in human testes. Guanidineacetate-methyltranspherase proved to be stimulated in the testes of the sexually immature rats in vivo under the action of HCGT on the 19th day after birth and from the 24th to the 27th day after birth. The activity of the enzyme was also increased in adult rats weighing 35--40 g. The activity of guanidineacetate-methyltranspherase in the testes of rats was particularly sharply stimulated after a single administration of N6-O2'-dibutiryladenosine-3',5'-cyclophosphate against the background of a preliminary treatment of rats with HCGT for a period of 5 days. The enzyme stimulation induced by combined treatment of rats with HCGT and N6-O2'-dibutiryladenosine-3',5'-cyclophosphate was prevented by the administration of cycloheximide and actinomycine D. The activity of the enzyme decreased after the incubation of the rat testes tissue homogenate with HCGT.
研究了人绒毛膜促性腺激素（HCGT）对人睾丸中胍乙酸-N-甲基转移酶活性的影响。结果表明，在出生后第19天以及出生后第24至27天，给性未成熟大鼠体内注射HCGT后，其睾丸中的胍乙酸甲基转移酶受到刺激。体重35 - 40克的成年大鼠的该酶活性也有所增加。在对大鼠进行为期5天的HCGT预处理后，单次注射N6-O2'-二丁酰腺苷-3',5'-环磷酸酯，大鼠睾丸中胍乙酸甲基转移酶的活性受到特别强烈的刺激。用环己酰亚胺和放线菌素D处理可阻止HCGT与N6-O2'-二丁酰腺苷-3',5'-环磷酸酯联合处理诱导的酶刺激。将大鼠睾丸组织匀浆与HCGT孵育后，该酶的活性降低。
