一个RNA发夹结构封闭了归巢内切核酸酶mobE基因的核糖体结合位点。
An RNA hairpin sequesters the ribosome binding site of the homing endonuclease mobE gene.
作者信息
Gibb Ewan A, Edgell David R
机构信息
Department of Biochemistry, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Canada.
出版信息
J Bacteriol. 2009 Apr;191(7):2409-13. doi: 10.1128/JB.01751-08. Epub 2009 Jan 30.
Abstract
Previous transcript mapping of the bacteriophage Aeh1 nrd operon revealed a predicted RNA hairpin upstream of the homing endonuclease mobE gene. We enzymatically mapped the hairpin, showing that the mobE ribosome binding site is sequestered. Cloning of the hairpin upstream of lacZ resulted in reduced beta-galactosidase activity, consistent with translational regulation.
摘要
先前对噬菌体Aeh1 nrd操纵子的转录本图谱分析显示，在归巢内切酶mobE基因上游存在一个预测的RNA发夹结构。我们通过酶切法对该发夹结构进行了图谱分析，结果表明mobE核糖体结合位点被封闭。将该发夹结构克隆到lacZ上游后，β-半乳糖苷酶活性降低，这与翻译调控一致。
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