Herring V L, Bastian T L, Lalonde R L
Department of Clinical Pharmacy, University of Tennessee, Memphis 38163.
J Chromatogr. 1991 Jun 14;567(1):221-7. doi: 10.1016/0378-4347(91)80325-7.
A method is described by which underivatized metoprolol enantiomers in plasma can be quantitated by high-performance liquid chromatography with fluorescence detection. Samples are prepared for injection using a simple solid-phase extraction procedure which is essentially 100% efficient for all analytes. The metoprolol enantiomers are resolved using a cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phase and a hexane-ethanol-diethylamine mobile phase. Samples were tested for adaptability to autoinjection and found to be stable for at least 16 h after reconstitution in mobile phase. The automated method was determined to be precise and accurate for enantiomer concentrations as low as 10 ng base per ml and was successfully employed in a pharmacokinetic investigation.
本文描述了一种方法，通过该方法可利用高效液相色谱-荧光检测法定量血浆中未衍生化的美托洛尔对映体。使用简单的固相萃取程序制备注射样品，该程序对所有分析物的萃取效率基本为100%。美托洛尔对映体采用三（3,5-二甲基苯基氨基甲酸酯）纤维素手性固定相和正己烷-乙醇-二乙胺流动相进行拆分。测试了样品对自动进样的适应性，发现其在流动相中复溶后至少16小时内稳定。该自动化方法对于低至每毫升10纳克碱基的对映体浓度测定具有精密度和准确性，并成功应用于一项药代动力学研究。
