Aboul-Enein H Y, Islam M R
Drug Development Laboratory, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
J Chromatogr. 1990 Jul 6;511:109-14. doi: 10.1016/s0021-9673(01)93275-3.
A high-performance liquid chromatographic method was developed for the direct resolution and optimization of the separation of timolol enantiomers. The method involves the use of a cellulose tris-3,5-dimethylphenylcarbamate chiral stationary phase (OD-Chiralcel) column. The effects of concentration of 2-propanol, various aliphatic alcohols and diethylamine in the mobile phase and column temperature on the retention and enantioselectivity of timolol enantiomers were studied. The maximum resolution factor obtained was 4.00 when using the solvent system hexane-2-propanol (95:5) containing 0.4% (v/v) diethylamine at 5 degrees C.
开发了一种高效液相色谱法，用于直接拆分和优化噻吗洛尔对映体的分离。该方法采用三（3,5-二甲基苯基氨基甲酸酯）纤维素手性固定相（OD-手性纤维素）柱。研究了流动相中异丙醇、各种脂肪醇和二乙胺的浓度以及柱温对噻吗洛尔对映体保留和对映选择性的影响。在5℃下使用含0.4%（v/v）二乙胺的己烷-异丙醇（95:5）溶剂系统时，获得的最大分离度因子为4.00。
