吞噬体内氧化爆发的强度和持续时间的测量。
Intraphagosomal measurement of the magnitude and duration of the oxidative burst.
作者信息
VanderVen Brian C, Yates Robin M, Russell David G
机构信息
Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA.
出版信息
Traffic. 2009 Apr;10(4):372-8. doi: 10.1111/j.1600-0854.2009.00877.x.
Abstract
Generation of an oxidative burst within the phagosomes of neutrophils, dendritic cells and macrophages is an essential component of the innate immune system. To examine the kinetics of the oxidative burst in the macrophage phagosome, we developed two new assays using beads coated with oxidation-sensitive fluorochromes.These assays permitted quantification and temporal resolution of the oxidative burst within the phagosome. The macrophage phagosomal oxidative burst is short lived,with oxidation of bead-associated substrates reaching maximal activity within 30 min following phagocytosis.Additionally, the extent and rate of macrophage phagosomal substrate oxidation were subject to immunomodulation by activation with lipopolysaccharide and/or interferon-gamma.
摘要
在中性粒细胞、树突状细胞和巨噬细胞的吞噬小体内产生氧化爆发是先天免疫系统的一个重要组成部分。为了检测巨噬细胞吞噬小体内氧化爆发的动力学，我们开发了两种新的检测方法，使用涂有氧化敏感荧光染料的珠子。这些检测方法能够对吞噬小体内的氧化爆发进行定量和时间分辨。巨噬细胞吞噬小体的氧化爆发持续时间较短，吞噬后30分钟内与珠子相关的底物氧化达到最大活性。此外，巨噬细胞吞噬小体底物氧化的程度和速率受到脂多糖和/或干扰素-γ激活的免疫调节。
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