Witte Florian, Dokas Janine, Neuendorf Franziska, Mundlos Stefan, Stricker Sigmar
Max Planck Institute for Molecular Genetics, Ihnestr. 73, D-14195 Berlin, Germany.
Gene Expr Patterns. 2009 Apr;9(4):215-23. doi: 10.1016/j.gep.2008.12.009. Epub 2009 Jan 13.
Wnt signalling plays important roles in patterning and outgrowth of the vertebrate limb. Different mutations in Wnt genes, their antagonists or (co-)receptors result in patterning and outgrowth defects as well as chondrocyte and bone phenotypes in mouse and human. Understanding Wnt activity during mouse limb development and chondrogenesis requires a temporal and spatial overview of Wnt signalling key factor expression. Here we present a comparative expression analysis of all 19 Wnt genes and their major secreted antagonists of the Dickkopf (Dkk), Wisp and the secreted frizzled related protein (Sfrp) families during mouse limb development. Our study reveals new domains of expression for Wnt2, Wnt2b, Wnt5b, Wnt6, Wnt7b, Wnt9a, Wnt10a, Wnt10b, Wnt11 and Wnt16, in the limb. We also identified novel expression domains for the Wnt antagonists Sfrp1, Sfrp3, Sfrp5, Wisp1 as well as Dkk2 and Dkk3. We provide a full expression pattern for Wif1 in limb development, for which no limb expression had been documented so far.
Wnt信号通路在脊椎动物肢体的模式形成和生长过程中发挥着重要作用。Wnt基因、其拮抗剂或（共）受体的不同突变会导致小鼠和人类出现模式形成和生长缺陷，以及软骨细胞和骨骼表型。要了解小鼠肢体发育和软骨形成过程中的Wnt活性，需要对Wnt信号关键因子的表达进行时空概述。在此，我们展示了在小鼠肢体发育过程中，对所有19个Wnt基因及其Dickkopf（Dkk）、Wisp和分泌型卷曲相关蛋白（Sfrp）家族的主要分泌拮抗剂进行的比较表达分析。我们的研究揭示了Wnt2、Wnt2b、Wnt5b、Wnt6、Wnt7b、Wnt9a、Wnt10a、Wnt10b、Wnt11和Wnt16在肢体中的新表达域。我们还确定了Wnt拮抗剂Sfrp1、Sfrp3、Sfrp5、Wisp1以及Dkk2和Dkk3的新表达域。我们提供了Wif1在肢体发育中的完整表达模式，迄今为止尚无其在肢体中表达的记录。
