Xu Yan, Xue Yang, Wang Ying, Feng Dechun, Lin Shuting, Xu Lingyun
Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, PR China.
Int Immunopharmacol. 2009 Mar;9(3):360-5. doi: 10.1016/j.intimp.2009.01.002. Epub 2009 Feb 3.
Microglial activation has been implicated in many neurological disorders for its inflammatory and/or neurotrophic effects. In this study, we investigated the effects upon activated microglia of Oridonin (Ori), an effective component isolated from Rabdosia rubescens. We pretreated rat primary microglia with or without Ori, then stimulated with lipopolysaccharide (LPS). We found that Ori pretreatment inhibited the release of proinflammatory mediators including nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1beta) and interleukin-6 (IL-6). Such suppressive effects were accompanied by inhibition of DNA binding activity of the critical transcription factor nuclear factor-kappa B (NF-kappaB). In addition, Ori upregulated the production of the neurotrophic factor, nerve growth factor (NGF). Our findings suggest that Ori may have anti-inflammatory and neuroregulatory effects through modulation of multiple functions of microglia.
小胶质细胞激活因其炎症和/或神经营养作用而与许多神经系统疾病有关。在本研究中，我们研究了冬凌草甲素（Ori）对激活的小胶质细胞的影响，冬凌草甲素是从冬凌草中分离出的一种有效成分。我们用或不用冬凌草甲素预处理大鼠原代小胶质细胞，然后用脂多糖（LPS）刺激。我们发现，冬凌草甲素预处理可抑制包括一氧化氮（NO）、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）在内的促炎介质的释放。这种抑制作用伴随着关键转录因子核因子-κB（NF-κB）DNA结合活性的抑制。此外，冬凌草甲素上调了神经营养因子神经生长因子（NGF）的产生。我们的研究结果表明，冬凌草甲素可能通过调节小胶质细胞的多种功能而具有抗炎和神经调节作用。
