MD. Nephrology Department - Shanxi Bethune Hospital - Shanxi Academy of Medical Sciences - Tongji Shanxi Hospital - Third Hospital of Shanxi Medical University - Taiyuan, and Syndrome Laboratory of Integrated of Chinese and Western Medicine - School of Chinese Medicine - Southern Medical University - Guangzhou, China.
MD. Department of Traditional Chinese Medicine - Xinyu People's Hospital - Xinyu, China.
Acta Cir Bras. 2022 May 2;37(2):e370204. doi: 10.1590/acb370204. eCollection 2022.
To evaluate the protective effect of Cuscuta chinensis Lam. polysaccharides (PCCL) on 5-fluorouracil-(5-FU)-induced intestinal mucositis (IM) in mice.
PCCL was orally administered at a dose of 20 mg·kg-1 for 7 days and its protective effect on 5-FU-induced IM (5-FU, 50 mg·kg-1 for 5 days) was evaluated by monitoring changes in body weight, degree of diarrhea, levels of tissue inflammatory factors (tumor necrosis factor α, interleukin 6, and interleukin 1β levels), apoptosis rates, and the expression levels of caspase-3, Bax and Bcl-2.
The severity of mucosal injury (as reflected by body weight changes, degree of diarrhea, height of villi, and damage to crypts) was significantly attenuated by PCCL administration. PCCL also reduced the levels of tissue inflammatory factors, the apoptosis rate, and the expression of caspase-3 and Bax, and increased Bcl-2 expression.
PCCL administration may be significantly protective against 5-FU-induced IM by inhibiting apoptosis and regulating the abnormal inflammation associated with it.
评价菟丝子多糖（PCCL）对 5-氟尿嘧啶（5-FU）诱导的小鼠肠黏膜炎（IM）的保护作用。
PCCL 以 20mg·kg-1 的剂量灌胃给药 7 天，通过监测体重变化、腹泻程度、组织炎症因子（肿瘤坏死因子-α、白细胞介素 6 和白细胞介素 1β 水平）、细胞凋亡率以及 caspase-3、Bax 和 Bcl-2 的表达水平，评价其对 5-FU 诱导的 IM（5-FU，50mg·kg-1，连用 5 天）的保护作用。
PCCL 给药可显著减轻黏膜损伤的严重程度（反映在体重变化、腹泻程度、绒毛高度和隐窝损伤）。PCCL 还降低了组织炎症因子水平、细胞凋亡率以及 caspase-3 和 Bax 的表达，并增加了 Bcl-2 的表达。
PCCL 给药可能通过抑制细胞凋亡和调节与其相关的异常炎症，对 5-FU 诱导的 IM 具有显著的保护作用。
