Awad Tamer, Belal Tarek, DeRuiter Jack, Kramer Kevin, Clark C Randall
Department of Pharmacal Sciences, Harrison School of Pharmacy, Auburn University, Auburn, AL 36849, USA.
Forensic Sci Int. 2009 Mar 10;185(1-3):67-77. doi: 10.1016/j.forsciint.2008.12.014. Epub 2009 Jan 30.
Gas chromatography-mass spectrometry (GC-MS) and gas chromatography-infrared detection (GC-IRD) methods were developed and compared for the differentiation of regioisomeric phenethylamines related to methamphetamine. There are a total of five regioisomeric phenethylamines (methamphetamine and four regioisomers) that produce essentially equivalent mass spectra. This unique set of five phenethylamines having the same molecular weight and elemental composition yield major mass spectral fragments at equivalent mass. The trifluoroacetyl derivatives of the primary and secondary amines yield characteristic individual fragment ions allowing structural differentiation among these regioisomers. The vapor phase infrared spectra generated via capillary gas chromatography differentiated among these compounds without the need for derivatization. The regioisomeric phenethylamines are well resolved by GC with the elution order generally determined by the degree of molecular linearity.
开发了气相色谱 - 质谱联用（GC-MS）和气相色谱 - 红外检测（GC-IRD）方法，并对其进行比较，以区分与甲基苯丙胺相关的区域异构体苯乙胺。共有五种区域异构体苯乙胺（甲基苯丙胺和四种区域异构体）产生基本等效的质谱图。这组独特的五种苯乙胺具有相同的分子量和元素组成，在等效质量处产生主要的质谱碎片。伯胺和仲胺的三氟乙酰衍生物产生特征性的单个碎片离子，从而能够区分这些区域异构体的结构。通过毛细管气相色谱产生的气相红外光谱无需衍生化即可区分这些化合物。这些区域异构体苯乙胺通过气相色谱得到很好的分离，洗脱顺序通常由分子线性程度决定。
