探究酵母电压依赖性阴离子通道1（VDAC1）在体内的方向。
Probing the orientation of yeast VDAC1 in vivo.
作者信息
McDonald Beth M, Wydro Mateusz M, Lightowlers Robert N, Lakey Jeremy H
机构信息
The Institute for Cell and Molecular Biosciences, Newcastle University, Framlington Place, Newcastle-upon-Tyne NE2 4HH, UK.
出版信息
FEBS Lett. 2009 Feb 18;583(4):739-42. doi: 10.1016/j.febslet.2009.01.039. Epub 2009 Jan 29.
Voltage dependent anion channel (VDAC) is a vital ion channel in mitochondrial outer membranes and its structure was recently shown to be a 19 stranded beta-barrel. However the orientation of VDAC in the membrane remains unclear. We probe here the topology and membrane orientation of yeast Saccharomyces cerevisiae in vivo. Five FLAG-epitopes were independently inserted into scVDAC1 and their surface exposure in intact and disrupted mitochondria detected by immunoprecipitation. Functionality was confirmed by measurements of respiration. Two epitopes suggest that VDAC (scVDAC) has its C-terminus exposed to the cytoplasm whilst two others are more equivocal and, when combined with published data, suggest a dynamic behavior.
电压依赖性阴离子通道（VDAC）是线粒体外膜中的一种重要离子通道，其结构最近被证明是一个由19条链组成的β桶状结构。然而，VDAC在膜中的取向仍不清楚。我们在此探究酿酒酵母中VDAC在体内的拓扑结构和膜取向。将五个FLAG表位独立插入酿酒酵母VDAC1（scVDAC1）中，并通过免疫沉淀检测它们在完整和破碎线粒体中的表面暴露情况。通过呼吸测量来确认其功能。两个表位表明VDAC（scVDAC）的C端暴露于细胞质中，而另外两个则更不明确，结合已发表的数据表明其具有动态行为。
Zotero 插件