Sampson M J, Lovell R S, Davison D B, Craigen W J
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
Genomics. 1996 Aug 15;36(1):192-6. doi: 10.1006/geno.1996.0445.
Voltage-dependent anion channels (VDACs) are small pore-forming channels found in the outer membrane of mitochondria. VDACs translocate adenine nucleotides and are the binding sites for several cytosolic kinases important in intermediary metabolism. Recently two human VDAC cDNAs (HVDAC1 and HVDAC2) were isolated, and possible orthologues of these genes have been isolated from mouse, bovine, and rat tissues. We report the isolation of a novel third VDAC cDNA from the mouse, designated MVDAC3. The deduced MVDAC3 protein is approximately 70% identical to the previously isolated MVDAC1 and MVDAC2 proteins. The MVDAC3 gene was mapped by an interspecies backcross panel to mouse chromosome 8. A database search using the mouse VDACs identified a second yeast VDAC-like gene that retains about 20% amino acid sequence identity with the mouse VDAC genes and 50% identity with the previously isolated yeast VDAC gene. The phylogenetic relationship of the eukaryotic VDAC genes is presented.
电压依赖性阴离子通道（VDACs）是在线粒体外膜中发现的形成小孔的通道。VDACs转运腺嘌呤核苷酸，并且是中间代谢中几种重要的胞质激酶的结合位点。最近分离出了两个人类VDAC cDNA（HVDAC1和HVDAC2），并且已经从小鼠、牛和大鼠组织中分离出了这些基因可能的直系同源物。我们报告从小鼠中分离出一种新的第三个VDAC cDNA，命名为MVDAC3。推导的MVDAC3蛋白与先前分离的MVDAC1和MVDAC2蛋白约70%相同。通过种间回交群体将MVDAC3基因定位到小鼠8号染色体上。使用小鼠VDACs进行数据库搜索，鉴定出第二个酵母VDAC样基因，该基因与小鼠VDAC基因保留约20%的氨基酸序列同一性，与先前分离的酵母VDAC基因有50%的同一性。展示了真核生物VDAC基因的系统发育关系。
