Ramunno-Johnson D, Strimbu C E, Fredrickson L, Arisaka K, Bozovic D
Department of Physics and Astronomy and California Nanosytems Institute University of California, Los Angeles, California 90095, USA.
Biophys J. 2009 Feb;96(3):1159-68. doi: 10.1016/j.bpj.2008.09.060.
Under in vitro conditions, free-standing hair bundles of the bullfrog (Rana catesbeiana) sacculus have exhibited spontaneous oscillations. We used a high-speed complementary metal oxide semiconductor camera to track the active movements of multiple hair cells in a single field of view. Our techniques enabled us to probe for correlations between pairs of cells, and to acquire records on over 100 actively oscillating bundles per epithelium. We measured the statistical distribution of oscillation periods of cells from different areas within the sacculus, and on different epithelia. Spontaneous oscillations exhibited a peak period of 33 ms (+29 ms, -14 ms) and uniform spatial distribution across the sacculus.
在体外条件下，牛蛙（牛蛙）球囊的独立毛束表现出自发振荡。我们使用高速互补金属氧化物半导体相机在单个视野中跟踪多个毛细胞的主动运动。我们的技术使我们能够探究细胞对之间的相关性，并获取每个上皮组织中超过100个主动振荡束的记录。我们测量了球囊内不同区域以及不同上皮组织中细胞振荡周期的统计分布。自发振荡的峰值周期为33毫秒（+29毫秒，-14毫秒），并且在球囊上呈均匀的空间分布。
