慢性牙周炎患者进展期牙周病变中γ-干扰素和转录因子T-bet的水平

Levels of interferon-gamma and transcription factor T-bet in progressive periodontal lesions in patients with chronic periodontitis.

作者信息

Dutzan Nicolas, Vernal Rolando, Hernandez Marcela, Dezerega Andrea, Rivera Oriana, Silva Nora, Aguillon Juan Carlos, Puente Javier, Pozo Patricia, Gamonal Jorge

机构信息

Periodontal Biology Laboratory, Department of Conservative Dentistry, Dentistry Faculty, University of Chile, Santiago, Chile.

出版信息

J Periodontol. 2009 Feb;80(2):290-6. doi: 10.1902/jop.2009.080287.

DOI:10.1902/jop.2009.080287

PMID:19186970

Abstract

BACKGROUND

Periodontitis is an infection with an episodic pattern of tissue-support destruction. During the generation of a primary CD4(+) T helper 1 (Th1) response, interferon-gamma (IFN-gamma) acts as a positive regulator by selectively inducing Th1 differentiation through increased transcription of T-bet. The aims of this work were to determine IFN-gamma levels in samples of gingival crevicular fluid (GCF) and to determine IFN-gamma and transcription factor T-bet expression in gingival tissue from patients undergoing the progression of chronic periodontitis.

METHODS

One hundred six patients with moderate or advanced chronic periodontitis were selected. Periodontitis was characterized by at least six sites with probing depth >or=5 mm, clinical attachment loss >or=3 mm, and radiographic bone loss. Periodontitis progression was determined by the tolerance method. GCF was collected using a paper strip, and enzyme-linked immunosorbent assay was performed to determine the total amount of IFN-gamma. Gingival biopsies were obtained from patients for real-time reverse transcription-polymerase chain reaction to determine IFN-gamma and T-bet expression. Statistical analysis was performed using statistical software. Data were expressed as subject means +/- SD. The chi(2) and Student t tests were used.

RESULTS

The total amount and concentration of cytokine IFN-gamma were significantly higher in active sites than in inactive sites (99.90 versus 68.90 pg; P = 0.03; 106.62 pg/mg versus 75.64 pg/mg, P = 0.04, respectively). Active sites showed a significantly lower Delta cycle threshold (Ct) of IFN-gamma than inactive sites (P = 0.04), whereas the expression of transcription factor T-bet was increased 1.42-fold in active sites compared to inactive sites.

CONCLUSION

The total amount and concentration of cytokine IFN-gamma in GCF samples and transcription factor T-bet expression were increased in progressive periodontal lesions in patients with chronic periodontitis.

摘要

背景

牙周炎是一种具有组织支持结构破坏发作模式的感染性疾病。在初始CD4(+)辅助性T细胞1（Th1）应答产生过程中，干扰素-γ（IFN-γ）通过增加T-bet转录选择性诱导Th1分化，从而起到正向调节作用。本研究旨在测定龈沟液（GCF）样本中IFN-γ水平，并确定慢性牙周炎进展期患者牙龈组织中IFN-γ和转录因子T-bet的表达情况。

方法

选取106例中度或重度慢性牙周炎患者。牙周炎的特征为至少6个位点探诊深度≥5mm、临床附着丧失≥3mm以及影像学骨丧失。采用耐受法确定牙周炎进展情况。使用纸条收集GCF，并通过酶联免疫吸附测定法测定IFN-γ总量。从患者获取牙龈活检组织，采用实时逆转录-聚合酶链反应测定IFN-γ和T-bet表达。使用统计软件进行统计分析。数据以受试者均值±标准差表示。采用卡方检验和学生t检验。

结果

活动位点细胞因子IFN-γ的总量和浓度显著高于非活动位点（分别为99.90对68.90 pg；P = 0.03；106.62 pg/mg对75.64 pg/mg，P = 0.04）。活动位点IFN-γ的Δ循环阈值（Ct）显著低于非活动位点（P = 0.04），而与非活动位点相比，活动位点转录因子T-bet的表达增加了1.42倍。