评估市售接触碟用于监测微生物环境的有效性。
Evaluation of the effectiveness of commercially available contact plates for monitoring microbial environments.
作者信息
Pinto F, Hiom S, Girdlestone S, Maillard J-Y
机构信息
Welsh School of Pharmacy, Cardiff University, Cardiff, UK.
出版信息
Lett Appl Microbiol. 2009 Mar;48(3):379-82. doi: 10.1111/j.1472-765X.2008.02534.x. Epub 2009 Feb 2.
AIM
The aim of this study was to measure the efficiency of contact plates to recover microbial contaminants from stainless steel surface.
MATERIALS AND METHODS
Three commercially available contact plates were used to recover two biological indicators from stainless steel sheets. The method used was standardised and validated to provide robust results. Parameters such as wetness, fertility and loss of water were also investigated for possible correlation with recovery efficiency.
RESULTS
The percentage of recovery from the contact plates was low and differences in recovery efficiency between brands depended upon the test organism. The poor recovery was probably due to the inability of the dried micro-organism to transfer to the plate, rather than the inability of the plate to grow the micro-organism. Wetness might help in improving recovery.
CONCLUSIONS
The use of a validated protocol allowed robust investigations into the recovery efficiency of contact plates.
SIGNIFICANCE AND IMPACT OF THE STUDY
The poor and variable recovery rates are of concern as they cast doubt on the comparability and reliability of environmental monitoring results where different commercial contact plates have been used.
目的
本研究旨在测定接触碟从不锈钢表面回收微生物污染物的效率。
材料与方法
使用三种市售接触碟从不锈钢片上回收两种生物指示剂。所采用的方法经过标准化和验证，以提供可靠的结果。还研究了诸如湿度、肥力和水分流失等参数与回收效率的可能相关性。
结果
接触碟的回收率较低，不同品牌之间回收效率的差异取决于测试微生物。回收率低可能是由于干燥的微生物无法转移到培养皿上，而不是培养皿无法使微生物生长。湿度可能有助于提高回收率。
结论
使用经过验证的方案能够对接触碟的回收效率进行可靠的研究。
研究的意义和影响
回收率低且变化不定令人担忧，因为这会让人怀疑在使用不同商业接触碟进行环境监测时结果的可比性和可靠性。
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