CIRAD, UMR Contrôle des Maladies, F-34398 Montpellier, France.
Comp Immunol Microbiol Infect Dis. 2010 Jul;33(4):279-90. doi: 10.1016/j.cimid.2008.08.011. Epub 2009 Feb 1.
Control of contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. mycoides Small Colony (MmmSC), remains an important goal in Africa. Subunit vaccines triggering B and T-cell responses could represent a promising approach. To this aim, the T-cell immunogenicity of four MmmSC lipoproteins (LppA, LppB, LppC and LppQ), present in African strains and able to elicit humoral response, was evaluated. In vitro assays revealed that only LppA was recognized by lymph node lymphocytes taken from three cattle, 3 weeks after MmmSC exposure. Maintenance of the LppA-specific response, relying on CD4 T-cells and IFN gamma production, was then demonstrated 1 year after infection. LppA is thus an important target for the CD4 T-cells generated early after MmmSC infection and persisting in the lymph nodes of recovered cattle. Its role as a protective antigen and ability to in vivo trigger both arms of the host immune response remain to be evaluated.
控制由绵羊肺炎支原体小菌落亚种（MmmSC）引起的传染性牛胸膜肺炎（CBPP）仍然是非洲的一个重要目标。引发 B 和 T 细胞反应的亚单位疫苗可能是一种很有前途的方法。为此，评估了四种存在于非洲株中并能引起体液反应的 MmmSC 脂蛋白（LppA、LppB、LppC 和 LppQ）的 T 细胞免疫原性。体外试验表明，只有 LppA 能被 MmmSC 暴露 3 周后的淋巴结淋巴细胞识别。在感染后 1 年，证明了依赖 CD4 T 细胞和 IFNγ产生的 LppA 特异性反应得以维持。因此，LppA 是 MmmSC 感染后早期产生并存在于恢复牛淋巴结中的 CD4 T 细胞的重要靶标。其作为保护性抗原的作用以及在体内触发宿主免疫反应两方面的能力仍有待评估。
