Totté Philippe, Rodrigues Valérie, Yaya Aboubakar, Hamadou Bamanga, Cisse Ousmane, Diallo Mahamadou, Niang Mamadou, Thiaucourt François, Dedieu Laurence
Cirad, UPR Contrôle des maladies, F-34398 Montpellier, France.
Vet Res. 2008 Jan-Feb;39(1):8. doi: 10.1051/vetres:2007046. Epub 2007 Nov 20.
A better understanding of protective immune memory against contagious bovine pleuropneumonia (CBPP) is needed in order to facilitate the development of safer vaccines based on selected components of the pathogen. For this purpose, cells collected from lymph nodes draining the lungs of Mycoplasma mycoides subsp. mycoides small colony biotype (MmmSC)-infected cattle were stimulated with the pathogen in vitro and evaluated concurrently for proliferation (CFSE based method), expression of activation, memory markers and cytokine production. Direct evidence is presented for a major contribution of CD4+ T cells to the vigorous proliferative and T1 biased cytokine recall responses observed in cattle that have recovered from infection but not in animals developing the acute form of the disease. Two different phenotypes of MmmSC-specific memory CD4 were observed based on CD62L expression and proliferative capacities. Furthermore, recall proliferation of B cells also occurred but was strictly dependent on the presence of CD4. The information provided in this study will facilitate the search for MmmSC antigens that have potential for the development of subunit vaccines against CBPP.
为了促进基于病原体特定成分的更安全疫苗的开发，需要更好地了解针对牛传染性胸膜肺炎（CBPP）的保护性免疫记忆。为此，从感染了丝状支原体丝状亚种小菌落生物型（MmmSC）的牛的肺部引流淋巴结中收集细胞，在体外用病原体进行刺激，并同时评估其增殖情况（基于CFSE的方法）、活化表达、记忆标志物和细胞因子产生。有直接证据表明，CD4 + T细胞对从感染中恢复的牛中观察到的强烈增殖和T1偏向性细胞因子回忆反应有主要贡献，但在发展为急性疾病形式的动物中则没有。基于CD62L表达和增殖能力，观察到两种不同表型的MmmSC特异性记忆CD4。此外，B细胞的回忆增殖也会发生，但严格依赖于CD4的存在。本研究提供的信息将有助于寻找具有开发抗CBPP亚单位疫苗潜力的MmmSC抗原。
