Laboratorio de Endocrinología de peces, Unidad de Morfología y Función, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios # 1, Los Reyes Iztacacala, 54090, Tlalnepantla, Edo. de México, México.
Laboratorio de Inmunología, Unidad de Morfología y Función, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. de lo Barrios # 1, Los Reyes Iztacala, 54090, Tlalnepantla, Edo. de México, México.
Fish Physiol Biochem. 2021 Feb;47(1):33-47. doi: 10.1007/s10695-020-00891-9. Epub 2020 Oct 29.
Reproduction in vertebrates is a complex process regulated by many hormones, and by paracrine factors and their receptors. This study aimed to examine the expression of pjGonadotropin-releasing hormone (GnRH 1), the kisspeptin receptor 2 (kissr2), and estradiol receptors α and β (ER α and ER β) during different stages of the sexual cycle and their distribution within the anterior brain of females of Chirostoma humboldtianum. Among these molecules, the kissr2 showed the maximal variation in expression, while GnRH 1 showed minimal variation of expression, and ERβ and ERα had intermediate variation of expression. The distribution of these molecules in the anterior brain was consistent with their levels of expression; kissr2 was widely distributed throughout the telencephalon and diencephalon, while ER and GnRH 1 showed more restricted distributions. No coexpression of kissr2 and ER in GnRH 1ergic neurons, suggesting that regulation of this GnRH variant is indirectly mediated by kisspeptin and estradiol.
脊椎动物的繁殖是一个受多种激素、旁分泌因子及其受体调节的复杂过程。本研究旨在探讨 Chirostoma humboldtianum 雌性个体在性周期不同阶段 pj 促性腺激素释放激素 (GnRH 1)、促性腺激素释放激素受体 2 (kissr2) 和雌激素受体 α 和 β (ER α 和 ER β) 的表达及其在前脑的分布。在这些分子中，kissr2 的表达变化最大，GnRH 1 的表达变化最小，而 ERβ 和 ERα 的表达变化处于中间水平。这些分子在前脑的分布与其表达水平一致；kissr2 在端脑和间脑广泛分布，而 ER 和 GnRH 1 的分布则较为局限。kissr2 和 ER 在 GnRH 1 能神经元中没有共表达，这表明这种 GnRH 变体的调节是间接由 kisspeptin 和雌二醇介导的。
