α-叶酸受体在上皮性卵巢癌及非肿瘤性卵巢组织中的表达

Alpha-folate receptor expression in epithelial ovarian carcinoma and non-neoplastic ovarian tissue.

作者信息

Markert Susanne, Lassmann Silke, Gabriel Boris, Klar Maximilian, Werner Martin, Gitsch Gerald, Kratz Felix, Hasenburg Annette

机构信息

Department of Obstetrics and Gynecology, University Medical Center, Hugstetter Strasse 55, Freiburg, Germany.

出版信息

Anticancer Res. 2008 Nov-Dec;28(6A):3567-72.

PMID:19189636

Abstract

BACKGROUND

The goal of this study was to evaluate the expression pattern and intracellular localization of alpha-folate receptor (alpha-FR) protein in human ovarian carcinoma compared with non-neoplastic ovarian tissue.

MATERIALS AND METHODS

Using immunohistochemistry (IHC), alpha-FR protein expression was analyzed in specimens of 104 human ovarian carcinomas and 30 non-neoplastic ovaries.

RESULTS

In 97% of the ovarian carcinomas, clear alpha-FR protein expression was detected (14% weak, 39% moderate, 44% strong). In the non-neoplastic ovaries, no (37%) or only weak (63%) expression was observed (p<0.0001). The tumor cells were characterized by a diffuse and homogeneous staining pattern. In tumor and non-tumor tissue, alpha-FR protein was detected predominantly in the cellular cytoplasm. In 41% of the ovarian carcinomas, cytoplasmic expression was localized towards the outer boarders of the invasive tumor cells and 30% exhibited additional nuclear alpha-FR protein expression.

CONCLUSION

Compared with nonneoplastic ovaries, alpha-FR protein is overexpressed in human ovarian carcinoma tissue.

摘要

背景

本研究的目的是评估与非肿瘤性卵巢组织相比，人卵巢癌中α-叶酸受体（α-FR）蛋白的表达模式和细胞内定位。

材料与方法

采用免疫组织化学（IHC）方法，分析了104例人卵巢癌标本和30例非肿瘤性卵巢标本中α-FR蛋白的表达情况。

结果

在97%的卵巢癌中检测到明显的α-FR蛋白表达（14%为弱阳性，39%为中度阳性，44%为强阳性）。在非肿瘤性卵巢中，未观察到表达（37%）或仅观察到弱阳性表达（63%）（p<0.0001）。肿瘤细胞的特征是弥漫性和均匀性染色模式。在肿瘤组织和非肿瘤组织中，α-FR蛋白主要在细胞质中检测到。在41%的卵巢癌中，细胞质表达定位于侵袭性肿瘤细胞的外缘，30%表现出额外的核α-FR蛋白表达。