Mitscherling Christoph, Maul Christof, Veselov Alexei A, Gericke Karl-Heinz
Institut für Physikalische und Theoretische Chemie, Technische Universität Braunschweig, Braunschweig, Germany.
Isotopes Environ Health Stud. 2009 Mar;45(1):59-67. doi: 10.1080/10256010802522200.
Laser-induced fluorescence (LIF) spectroscopy is an efficient tool for the detection of low nitric oxide (NO) concentrations down to the parts per trillion (ppt) range. The isotopic selectivity of this method provides a broad potential of applications. Whereas (14)N(16)O and (15)N(16)O have been described extensively this is not the case for the (14)N(18)O isotopologue. A (1+1) resonance enhanced multiphoton excitation time-of-flight mass spectrometer has been used for the simultaneous detection of (14)N(16)O, (15)N(16)O and (14)N(18)O isotopologues. Regions with single (14)N(18)O transitions have been studied in more detail by the LIF device. Electronic excitation of the A-X transition in the UV provides fluorescence which is monitored around 247 nm from A(2)Sigma(+)(v'=0)-->X(2)Pi(Omega)(v''=2). This transition has been used for an isotope specific online detection of (14)N(18)O from exhaled air. The detection limit for (14)N(18)O is 7.3+/-1.8 ppt. With respect to (18)O-labelled drugs and amino acids this denotes a novel extension to NO research.
激光诱导荧光（LIF）光谱法是一种用于检测低至万亿分之一（ppt）浓度一氧化氮（NO）的有效工具。该方法的同位素选择性提供了广泛的应用潜力。虽然（14）N（16）O和（15）N（16）O已被广泛描述，但（14）N（18）O同位素分子并非如此。一台（1+1）共振增强多光子激发飞行时间质谱仪已被用于同时检测（14）N（16）O、（15）N（16）O和（14）N（18）O同位素分子。LIF装置对具有单个（14）N（18）O跃迁的区域进行了更详细的研究。紫外光中A-X跃迁的电子激发产生荧光，在247nm附近监测从A（2）Σ⁺（v'=0）→X（2）Π（Ω）（v''=2）的荧光。该跃迁已被用于对呼出气体中（14）N（18）O进行同位素特异性在线检测。（14）N（18）O的检测限为7.3±1.8ppt。对于（18）O标记的药物和氨基酸而言，这意味着NO研究的一个新扩展。
