伊朗中部人兽共患皮肤利什曼病病媒和储存宿主的分子数据。

Molecular data on vectors and reservoir hosts of zoonotic cutaneous leishmaniasis in central Iran.

作者信息

Rassi Y, Abai M R, Javadian E, Rafizadeh S, Imamian H, Mohebali M, Fateh M, Hajjaran H, Ismaili K

机构信息

Department of medical entomology & vector control, School of public health & Institute of health research, Tehran University of medical sciences, Tehran, Iran.

出版信息

Bull Soc Pathol Exot. 2008 Dec;101(5):425-8.

DOI:

PMID:19192615

Abstract

Due to the increasing number of positive cases of cutaneous leishmaniasis with occurrence of new foci, a study was carried out to investigate on vectors and reservoirs of the disease in the Shahrood district, central Iran during 2005-2006. Sandflies and rodents were collected using sticky papers and Sherman live traps respectively More than 1700 sandflies were collected and identified, mainly Phlebotomus papatasi species. RAPD-PCR analysis of sandflies showed that 10% of P. papatasi and 4.2% of P. caucasicus were naturally infected with Leishmania major. Two species of rodents, potential reservoirs, Rhombomys opimus (92.5%) and Nesokia indica (7.5%) were trapped in the district. Microscopy identification from rodents confirmed that 91.9% of the Rhombomys opimus were positive to amastigotes. Species identification of isolated parasites revealed Leishmania major DNA in the infected Rhombomys using RAPD-PCR technique. This epidemiological data highlight the importance of the disease in the region and could help people involved in control programs.

摘要

由于皮肤利什曼病确诊病例数不断增加且出现了新疫源地，2005 - 2006年期间在伊朗中部沙赫鲁德地区开展了一项关于该疾病病媒和宿主的研究。分别使用粘纸和谢尔曼活捕陷阱收集白蛉和啮齿动物。共收集并鉴定了1700多只白蛉，主要为巴氏白蛉。对白蛉的随机扩增多态性DNA聚合酶链反应（RAPD - PCR）分析表明，10%的巴氏白蛉和4.2%的高加索白蛉自然感染了硕大利什曼原虫。该地区捕获了两种可能作为宿主的啮齿动物，大沙鼠（92.5%）和印度地鼠（7.5%）。对啮齿动物的显微镜鉴定证实，91.9%的大沙鼠无鞭毛体呈阳性。使用RAPD - PCR技术对分离出的寄生虫进行物种鉴定，在受感染的大沙鼠中发现了硕大利什曼原虫DNA。这些流行病学数据凸显了该疾病在该地区的重要性，并可为参与防控计划的人员提供帮助。