Abai M A, Rassi Y, Imamian H, Fateh M, Mohebali M, Rafizadeh S, Hajjaran H, Azizi K, Ismaiili M
Department of Medical Entomology and Vector Control, School of Public Health and Institute of Health Research, Tehran University of Medical Sciences, Tehran, Iran.
Pak J Biol Sci. 2007 Jun 15;10(12):2061-5. doi: 10.3923/pjbs.2007.2061.2065.
A study was made in rural region of Shahrood city, Semnan province in the central of Iran during 2005 to investigate of vectors of zoonotic cutaneous leishmaniasis. Sticky traps and an aspirator were used for collecting of sandflies. Three species of Phlebotomus papatasi, P. caucasicus and Sergentomyia sintoni were collected and identified and the first species was dominant (53%). Nested PCR method were employed for identifying of isolated parasites of dissected female of sandflies. Among the dissected sand flies 3 out of 24 (12.5%) Phlebotomus papatasi and 2 out of 48 (4.2%) Phlebotomus caucasicus were found naturally infected with promastigotes. Species-specific amplification ofGiemsa staind promastigote slides revealed specific PCR production of Leishmania major DNA in the infected P. papatasi and P. caucasicus sand flies. Having high prevalence and infection rate provide enough evidence to incriminate of P. papatasi as the main and proven vector of cutaneous leishmaniasis to human in the region and the species of P. caucasicus play the second role for maintenance of disease between rodents.
2005年，在伊朗中部塞姆南省沙赫鲁德市的农村地区开展了一项研究，以调查人兽共患皮肤利什曼病的病媒。使用粘性诱捕器和吸虫器收集白蛉。共收集并鉴定出三种白蛉，即巴氏白蛉、高加索白蛉和辛氏司蛉，其中第一种白蛉占主导地位（53%）。采用巢式PCR方法鉴定解剖的雌性白蛉体内分离出的寄生虫。在解剖的白蛉中，24只巴氏白蛉中有3只（12.5%）、48只高加索白蛉中有2只（4.2%）被发现自然感染前鞭毛体。对吉姆萨染色的前鞭毛体玻片进行种特异性扩增，结果显示在受感染的巴氏白蛉和高加索白蛉中，利什曼原虫主要DNA有特异性PCR产物。高流行率和感染率提供了充分的证据，证明巴氏白蛉是该地区人类皮肤利什曼病的主要且已证实的病媒，而高加索白蛉在啮齿动物之间维持疾病传播方面起次要作用。
