伊朗人兽共患皮肤利什曼病流行区自然感染杜氏利什曼原虫和热带利什曼原虫的首次报告
First Report of Natural Infection of to and in the Endemic Foci of Zoonotic Cutaneous Leishmaniasis in Iran.
作者信息
Absavaran Azad, Mohebali Mehdi, Moin-Vaziri Vahideh, Zahraei-Ramazani Alireza, Akhavan Amir Ahmad, Rafizadeh Sayena, Rassi Amirhossin, Barmaki Alireza, Rassi Yavar
机构信息
Department of Medical Entomology and Vector control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Medical entomology and Vector control, Zanjan University of Medical Sciences, Zanjan, Iran.
出版信息
J Arthropod Borne Dis. 2022 Dec 31;16(4):315-324. doi: 10.18502/jad.v16i4.12086. eCollection 2022 Dec.
BACKGROUND
The primary aim of this study is to determine infection to parasites in the wild population of and using molecular methods in some important zoonotic cutaneous leishmaniasis foci in Iran.
METHODS
Sand flies were collected from active colonies of rodent burrows from 16 trapping sites using sticky trap paper. In order to detect and identify of parasites in females and the Nested-PCR amplification of ITS2-rDNA region was performed to generate amplicon with 245bp for , 206bp for and 141bp for .
RESULTS
In the current study we found DNA of different gerbil parasites such as and and mixed infection of in and It should be noted that, in Iran, natural infection with parasites is recorded for the first time in this study in .
CONCLUSION
Both species of and not only may participate in the ZCL transmission cycle between reservoir hosts, but also results of this study support the role of these species as secondary vectors in the transmission of leishmaniasis to humans.
背景
本研究的主要目的是利用分子方法，在伊朗一些重要的人兽共患皮肤利什曼病疫源地，确定野生 和 种群中的寄生虫感染情况。
方法
使用粘性诱捕纸从16个诱捕地点的啮齿动物洞穴活动群落中收集白蛉。为了检测和鉴定雌性 和 体内的寄生虫，对ITS2-rDNA区域进行巢式PCR扩增，以产生 的245bp、 的206bp和 的141bp扩增子。
结果
在本研究中，我们发现了不同沙鼠寄生虫的DNA，如 和 ，以及 和 中的混合感染。应该指出的是，在伊朗，本研究首次在 中记录到 寄生虫的自然感染。
结论
和 这两个物种不仅可能参与保虫宿主之间的皮肤利什曼病传播循环，而且本研究结果支持了这些物种作为利什曼病传播给人类的次要媒介的作用。
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