单点突变导致使用Cobas TaqMan HIV-1实时荧光定量PCR检测法对1型人类免疫缺陷病毒（HIV-1）载量的低估超过100倍。
Single-point mutations causing more than 100-fold underestimation of human immunodeficiency virus type 1 (HIV-1) load with the Cobas TaqMan HIV-1 real-time PCR assay.
作者信息
Korn Klaus, Weissbrich Benedikt, Henke-Gendo Cornelia, Heim Albert, Jauer Christin Mariel, Taylor Ninon, Eberle Josef
机构信息
Institute of Clinical and Molecular Virology, National Reference Center for Retroviruses, University of Erlangen-Nuremberg, Erlangen, Germany.
出版信息
J Clin Microbiol. 2009 Apr;47(4):1238-40. doi: 10.1128/JCM.02204-08. Epub 2009 Feb 4.
Abstract
Systematic sequence analysis of human immunodeficiency virus type 1 (HIV-1) variants with RNA levels underestimated by the Cobas TaqMan HIV-1 assay demonstrated that mutations at a single position of the downstream primer can lead to the underestimation of HIV-1 RNA levels by more than 2 log and to false-negative results in minipool screening of blood donors. Mutations at this position are found in about 2% of all HIV-1 M gag sequences.
摘要
对人类免疫缺陷病毒1型（HIV-1）变异体进行系统序列分析，这些变异体的RNA水平被Cobas TaqMan HIV-1检测法低估，结果表明下游引物单个位置的突变可导致HIV-1 RNA水平被低估超过2个对数，并在献血者混合样本筛查中产生假阴性结果。在所有HIV-1 M gag序列中约2%发现了该位置的突变。
相似文献
1
Single-point mutations causing more than 100-fold underestimation of human immunodeficiency virus type 1 (HIV-1) load with the Cobas TaqMan HIV-1 real-time PCR assay.
J Clin Microbiol. 2009 Apr;47(4):1238-40. doi: 10.1128/JCM.02204-08. Epub 2009 Feb 4.
2
Mutations to the probe of Cobas TaqMan HIV-1 ver. 1.0 assay causing undetectable viral load in a patient with acute HIV-1 infection.
J Infect Chemother. 2011 Dec;17(6):863-5. doi: 10.1007/s10156-011-0263-9. Epub 2011 Jun 14.
3
Human immunodeficiency virus type 1 (HIV-1) plasma load discrepancies between the Roche COBAS AMPLICOR HIV-1 MONITOR Version 1.5 and the Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 assays.
J Clin Microbiol. 2007 Oct;45(10):3436-8. doi: 10.1128/JCM.00973-07. Epub 2007 Aug 22.
4
Fully automated quantification of human immunodeficiency virus (HIV) type 1 RNA in human plasma by the COBAS AmpliPrep/COBAS TaqMan system.
J Clin Virol. 2007 Apr;38(4):304-12. doi: 10.1016/j.jcv.2006.12.022. Epub 2007 Feb 27.
5
Improved Performance of COBAS AmpliPrep/COBAS TaqMan Version 2.0 Assay Over Amplicor Monitor Version 1.5 in the Quantification of HIV-1 RNA Viral Load in Abuja, Nigeria.
Curr HIV Res. 2015;13(4):262-7. doi: 10.2174/1570162x13666150121112139.
6
Comparison of Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 test version 2.0 (CAP/CTM v2.0) with other real-time PCR assays in HIV-1 monitoring and follow-up of low-level viral loads.
J Virol Methods. 2013 Jan;187(1):1-5. doi: 10.1016/j.jviromet.2012.10.004. Epub 2012 Oct 22.
7
Short communication: identification of a novel HIV type 1 subtype H/J recombinant in Canada with discordant HIV viral load (RNA) values in three different commercial assays.
AIDS Res Hum Retroviruses. 2007 Nov;23(11):1309-13. doi: 10.1089/aid.2007.0080.
8
Comparative RNA quantification of HIV-1 group M and non-M with the Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 v2.0 and Abbott Real-Time HIV-1 PCR assays.
J Acquir Immune Defic Syndr. 2011 Mar 1;56(3):239-43. doi: 10.1097/QAI.0b013e3182099891.
9
Performance of the new Roche Cobas AmpliPrep-Cobas TaqMan version 2.0 human immunodeficiency virus type 1 assay.
J Clin Microbiol. 2009 Oct;47(10):3400-2. doi: 10.1128/JCM.00727-09. Epub 2009 Aug 5.
10
Comparison of the LCx human immunodeficiency virus (HIV) RNA quantitative, RealTime HIV, and COBAS AmpliPrep-COBAS TaqMan assays for quantitation of HIV type 1 RNA in plasma.
J Clin Microbiol. 2006 Aug;44(8):2963-6. doi: 10.1128/JCM.00341-06.
引用本文的文献
1
Analysis of the Frequency of Mutations at Diagnostic Oligonucleotide Sites and Their Impact on the Efficiency of PCR for HIV-1.
Microorganisms. 2023 Nov 22;11(12):2838. doi: 10.3390/microorganisms11122838.
2
SARS-CoV-2 samples may escape detection because of a single point mutation in the N gene.
Euro Surveill. 2020 Oct;25(39). doi: 10.2807/1560-7917.ES.2020.25.39.2001650.
3
Nucleic acid testing and molecular characterization of HIV infections.
Eur J Clin Microbiol Infect Dis. 2019 May;38(5):829-842. doi: 10.1007/s10096-019-03515-0. Epub 2019 Feb 23.
4
Diagnosis of Human Immunodeficiency Virus Infection.
Clin Microbiol Rev. 2018 Nov 28;32(1). doi: 10.1128/CMR.00064-18. Print 2019 Jan.
5
Nucleoside analogs to manage sequence divergence in nucleic acid amplification and SNP detection.
Nucleic Acids Res. 2018 Jul 6;46(12):5902-5910. doi: 10.1093/nar/gky392.
6
Real-time PCR assays for hepatitis B virus DNA quantification may require two different targets.
Virol J. 2017 May 12;14(1):94. doi: 10.1186/s12985-017-0759-8.
7
Evaluation of the Procleix Ultrio Elite Assay and the Panther-System for Individual NAT Screening of Blood, Hematopoietic Stem Cell, Tissue and Organ Donors.
Transfus Med Hemother. 2016 May;43(3):177-82. doi: 10.1159/000446217. Epub 2016 May 3.
8
Comparison of the Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 test v1.0 with v2.0 in HIV-1 viral load quantification.
Kaohsiung J Med Sci. 2015 Apr;31(4):188-93. doi: 10.1016/j.kjms.2014.12.010. Epub 2015 Feb 3.
9
Development, validation and clinical evaluation of a low cost in-house HIV-1 drug resistance genotyping assay for Indian patients.
PLoS One. 2014 Aug 26;9(8):e105790. doi: 10.1371/journal.pone.0105790. eCollection 2014.
10
A robust HIV-1 viral load detection assay optimized for Indian sub type C specific strains and resource limiting setting.
Biol Res. 2014 May 30;47(1):22. doi: 10.1186/0717-6287-47-22.
本文引用的文献
1
Human immunodeficiency virus type 1 (HIV-1) plasma load discrepancies between the Roche COBAS AMPLICOR HIV-1 MONITOR Version 1.5 and the Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 assays.
J Clin Microbiol. 2007 Oct;45(10):3436-8. doi: 10.1128/JCM.00973-07. Epub 2007 Aug 22.
2
Impaired quantification of plasma HIV-1 RNA with a commercialized real-time PCR assay in a couple of HIV-1-infected individuals.
J Clin Virol. 2007 Jul;39(3):226-9. doi: 10.1016/j.jcv.2007.04.007. Epub 2007 May 25.
3
Rates of and reasons for failure of commercial human immunodeficiency virus type 1 viral load assays in Brazil.
J Clin Microbiol. 2007 Jun;45(6):2061-3. doi: 10.1128/JCM.00136-07. Epub 2007 Mar 28.
Zotero 插件