Laboratoire de Virologie, Hôpital Saint-Louis AP-HP, INSERM U941, Université Paris-Diderot, Paris, France.
J Acquir Immune Defic Syndr. 2011 Mar 1;56(3):239-43. doi: 10.1097/QAI.0b013e3182099891.
A new version of the Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 assay (CA/CTM v2.0) has been introduced to overcome the underquantification observed with the first version.
We compared the Roche Cobas CA/CTM v2.0 and Abbott RealTime HIV-1 assays for HIV-1 group M and non-M viral load measurement.
We found a good correlation (r = 0.96) between the 2 techniques for the 260 HIV-1 group M plasma samples tested. The Roche Cobas assay gave significantly higher values than the Abbott assay, and 51 samples (20%) yielded differences greater than 0.5 log10 copies per milliliter. Conversely, 2 samples were more than 0.5 log10 copies per milliliter higher with the Abbott assay than with the Roche Cobas assay. Among the 84 samples with undetectable viral load in the Abbott assay (detection limit 40 copies/mL), 17 (20%) were detectable with the CA/CTM v2.0 assay (detection limit 20 copies/mL), with values ranging from 41 to 897 copies per milliliter. Extrapolation of the Abbott curves led to 10/17 (59%) of these samples being quantifiable. HIV-1 groups O and P were similarly quantified by the two techniques.
The results of the Roche Cobas CA/CTM v2.0 and Abbott RealTime HIV-1 assays correlate well. The new version of the CA/CTM assay shows improved sensitivity. Nevertheless, the 2 assays differ by more than 0.5 log₁₀ copies per milliliter for some samples.
罗氏 Cobas AmpliPrep/Cobas TaqMan HIV-1 检测系统（CA/CTM v2.0）的新版本已被引入，以克服第一代检测系统存在的定量不足问题。
我们比较了罗氏 Cobas CA/CTM v2.0 和 Abbott RealTime HIV-1 检测系统用于 HIV-1 组 M 和非 M 病毒载量测量的性能。
我们发现，在检测的 260 份 HIV-1 组 M 血浆样本中，这两种技术具有良好的相关性（r = 0.96）。罗氏 Cobas 检测系统给出的结果显著高于 Abbott 检测系统，有 51 份样本（20%）的差异大于 0.5 log₁₀ 拷贝/毫升。相反，在 Abbott 检测系统中，有 2 份样本的结果比罗氏 Cobas 检测系统高出 0.5 log₁₀ 拷贝/毫升以上。在 Abbott 检测系统中不可检测到病毒载量的 84 份样本中（检测限为 40 拷贝/ml），17 份（20%）用 CA/CTM v2.0 检测系统检测到（检测限为 20 拷贝/ml），结果范围为 41 至 897 拷贝/ml。Abbott 曲线的外推导致这 17 份样本中的 10 份（59%）可定量。两种技术对 HIV-1 组 O 和 P 的定量结果相似。
罗氏 Cobas CA/CTM v2.0 和 Abbott RealTime HIV-1 检测系统的结果相关性良好。CA/CTM 检测系统的新版本显示出了更好的灵敏度。然而，对于一些样本，两种检测系统的结果差异超过 0.5 log₁₀ 拷贝/ml。
