栽培异源四倍体棉属物种及其二倍体祖先中类包膜结构域的序列异质性。

Sequence heterogeneity of the envelope-like domain in cultivated allotetraploid Gossypium species and their diploid progenitors.

作者信息

Hafez E E, Abdel Ghany A A, Paterson A H, Zaki E A

机构信息

Molecular plant pathology Department, Arid land research institute, Mubarak City for Research, Alexandria, Egypt.

出版信息

J Appl Genet. 2009;50(1):17-23. doi: 10.1007/BF03195647.

DOI:10.1007/BF03195647

PMID:19193978

Abstract

Retroviral envelope (env)-like sequences in 2 cultivated allotetraploid cottons and their diploid progenitors have been identified and characterized in this study. DNA sequence analysis reveals that these sequences are heterogeneous. The observed sequence diversity, however, seems to preserve coding information. This is evidenced by the detection of the transmembrane domain (TM), which is the most conserved feature of the divergent retroviral env genes. The high ratio of synonymous to nonsynonymous changes suggests that these sequences are evolving under purifying selection. Phylogenetic analysis shows that Gossypium sequences closely cluster with a lineage of plant endogenous retroviruses that have an env-like gene. These results provide evidence for the antiquity and the wide diversity of env-like sequences in the Gossypium genome.

摘要

在本研究中，已鉴定并表征了2种栽培异源四倍体棉花及其二倍体祖先中类似逆转录病毒包膜（env）的序列。DNA序列分析表明这些序列是异质的。然而，观察到的序列多样性似乎保留了编码信息。这通过跨膜结构域（TM）的检测得到证明，跨膜结构域是不同逆转录病毒env基因最保守的特征。同义变化与非同义变化的高比率表明这些序列在纯化选择下进化。系统发育分析表明，棉属序列与具有类似env基因的植物内源性逆转录病毒谱系紧密聚类。这些结果为棉属基因组中类似env序列的古老性和广泛多样性提供了证据。