来自柑橘溃疡病菌野油菜黄单胞菌柑橘致病变种的麦芽糖结合蛋白（MalE）的结晶、数据收集与数据处理
Crystallization, data collection and data processing of maltose-binding protein (MalE) from the phytopathogen Xanthomonas axonopodis pv. citri.
作者信息
Souza C S, Ferreira L C S, Thomas L, Barbosa J A R G, Balan A
机构信息
Departamento de Microbiologia, Instituto de Ciências Biomédicas II, Universidade de São Paulo, São Paulo, SP, Brazil.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Feb 1;65(Pt 2):105-7. doi: 10.1107/S1744309108041833. Epub 2009 Jan 7.
Abstract
Maltose-binding protein is the periplasmic component of the ABC transporter responsible for the uptake of maltose/maltodextrins. The Xanthomonas axonopodis pv. citri maltose-binding protein MalE has been crystallized at 293 K using the hanging-drop vapour-diffusion method. The crystal belonged to the primitive hexagonal space group P6(1)22, with unit-cell parameters a = 123.59, b = 123.59, c = 304.20 A, and contained two molecules in the asymetric unit. It diffracted to 2.24 A resolution.
摘要
麦芽糖结合蛋白是负责摄取麦芽糖/麦芽糊精的ABC转运蛋白的周质成分。柑橘溃疡病菌的麦芽糖结合蛋白MalE已采用悬滴气相扩散法在293 K下结晶。晶体属于原始六方空间群P6(1)22，晶胞参数a = 123.59，b = 123.59，c = 304.20 Å，不对称单元中包含两个分子。其衍射分辨率为2.24 Å。
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