Hinenoya Noriko, Naito Ichiro, Momota Ryusuke, Sado Yoshikazu, Kumagishi Kanae, Ninomiya Yoshifumi, Ohtsuka Aiji
Department of Human Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Arch Histol Cytol. 2008 Nov;71(3):185-94. doi: 10.1679/aohc.71.185.
In the present study, we have analyzed the alpha(IV) chain distribution in the subepithelial basement membrane (BM) of the rat pulmonary airway from the bronchi to alveoli. We have furthermore analyzed the alpha(IV) chain distribution in the subepithelial BM of the bronchioalveolar duct junction (BADJ) using alpha(IV) chain specific monoclonal antibodies. Our results show that the BM of the bronchial and bronchiolar epithelium contains [alpha1(IV)]2alpha2(IV) and [alpha5(IV)]2alpha6(IV) molecules and confirmed that the alveolar BM consists of [alpha1(IV)]2alpha2(IV) and alpha3(IV) alpha4(IV)alpha5(IV) molecules. There are also small regions in BADJ consisting of only [alpha1(IV)]2alpha2(IV) molecules without alpha3(IV)alpha4(IV)alpha5(IV) and [alpha5(IV)]2alpha6(IV) molecules. Moreover, the bronchioalveolar stem cells (BASCs)-primordial cells for bronchiolar Clara cells and alveolar type II (AT2) cells - lie adjacent to such small regions. These findings suggest that [alpha1(IV)]2 alpha2(IV) may be important for the BASCs to self-renew or to self-maintain themselves and that microenvironments produced by alpha(IV) chains may be important for cell differentiation.
在本研究中，我们分析了大鼠肺气道从支气管到肺泡的上皮下基底膜（BM）中α（IV）链的分布。我们还使用α（IV）链特异性单克隆抗体分析了支气管肺泡管连接处（BADJ）上皮下BM中α（IV）链的分布。我们的结果表明，支气管和细支气管上皮的BM含有[α1（IV）]2α2（IV）和[α5（IV）]2α6（IV）分子，并证实肺泡BM由[α1（IV）]2α2（IV）和α3（IV）α4（IV）α5（IV）分子组成。在BADJ中也有一些小区域仅由[α1（IV）]2α2（IV）分子组成，没有α3（IV）α4（IV）α5（IV）和[α5（IV）]2α6（IV）分子。此外，支气管肺泡干细胞（BASCs）——细支气管克拉拉细胞和肺泡II型（AT2）细胞的原始细胞——位于这些小区域附近。这些发现表明，[α1（IV）]2α2（IV）可能对BASCs的自我更新或自我维持很重要，并且由α（IV）链产生的微环境可能对细胞分化很重要。
