Key Laboratory for Bio-drugs of Ministry of Health, Research Center for Medicinal Biotechnology, Shandong Academy of Medical Sciences, 250062 Jinan, Shandong, People's Republic of China.
Rheumatol Int. 2009 Oct;29(12):1411-6. doi: 10.1007/s00296-009-0870-2. Epub 2009 Feb 8.
PADI4 that catalyzes the conversion of peptidylarginine to citrulline is associated with rheumatoid arthritis in some populations. The current study investigated the expressions of PADI4 in synovial fluid of RA (n = 73), osteoarthritis (OA, n = 96) and ankylosing spondylitis (AS, n = 32) using ELISA and western blotting following immuno-precipitation (n = 6 for each diseases). The study also compared the mRNA level of PADI4 in the synovial membrane of RA with the levels in the samples of OA and AS (n = 6 for each diseases) using real time PCR. ELISA detected a higher level of PADI4 in SF of RA than in samples of OA and AS (P = 0.0001). The level of PADI4 was significantly correlated with the level of rheumatic factor (P = 0.015), but not with anti cyclic citrullinated peptide antibody (anti-CCP) in the RA fluids. Western blotting confirmed the expression of PADI4 in SF of RA. Quantitative PCR measured higher transcription of PADI4 in the synovial membrane of RA than in the samples of OA and AS. The results confirmed increased expression of PADI4 in synovium of RA.
在某些人群中，催化肽精氨酸向瓜氨酸转化的 PADI4 与类风湿关节炎有关。本研究采用免疫沉淀后 ELISA 和 Western blot（每种疾病各 6 例）检测了滑膜液中 PADI4 在类风湿关节炎（n = 73）、骨关节炎（OA，n = 96）和强直性脊柱炎（AS，n = 32）中的表达。还采用实时 PCR 比较了 RA 滑膜膜中 PADI4 的 mRNA 水平与 OA 和 AS 样本中的水平（每种疾病各 6 例）。ELISA 检测到 RA 患者 SF 中的 PADI4 水平高于 OA 和 AS 样本（P = 0.0001）。PADI4 水平与 RA 液中的风湿因子水平显著相关（P = 0.015），但与抗环瓜氨酸肽抗体（抗-CCP）无关。Western blot 证实了 RA SF 中 PADI4 的表达。定量 PCR 测量到 RA 滑膜膜中 PADI4 的转录高于 OA 和 AS 样本。结果证实 RA 滑膜中 PADI4 的表达增加。
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